Platelet-rich plasma-containing fragmin-protamine micro-nanoparticles promote epithelialization and angiogenesis in split-thickness skin graft donor sites

Abstract Background Platelet-rich plasma (PRP) contains multiple growth factors, and fragmin-protamine micro-nanoparticles (F-P M-NPs) significantly enhance and stabilize growth factors. The purpose of this study was to evaluate the effects of PRP-containing F-P M-NPs (PRP&F-P M-NPs) on wound re...

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Bibliographic Details
Published in:The Journal of surgical research 2015-01, Vol.193 (1), p.483-491
Main Authors: Takabayashi, Yuki, MD, Ishihara, Masayuki, PhD, Sumi, Yuki, MD, Takikawa, Makoto, MD, Nakamura, Shingo, PhD, Kiyosawa, Tomoharu, MD, PhD
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Dalteparin - pharmacology
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelialization
Fragmin-protamine micro-nanoparticles
Injections, Intradermal
Male
Nanoparticles
Neovascularization, Physiologic - drug effects
Platelet-Rich Plasma
Protamines - pharmacology
Rats, Inbred F344
Regeneration
Skin - blood supply
Skin - cytology
Skin - injuries
Skin Transplantation - methods
Split-thickness skin graft donor sites
Surgery
Treatment Outcome
Wound Healing - drug effects
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Summary:Abstract Background Platelet-rich plasma (PRP) contains multiple growth factors, and fragmin-protamine micro-nanoparticles (F-P M-NPs) significantly enhance and stabilize growth factors. The purpose of this study was to evaluate the effects of PRP-containing F-P M-NPs (PRP&F-P M-NPs) on wound repair in split-thickness skin graft (STSG-) donor sites (DS). Materials and methods A total of 56 inbred male rats were anesthetized and split-thickness skin graft donor site (STSG-DS) were created with a Padgett dermatome. PRP&F-P M-NPs, F-P M-NPs, PRP, and saline (control) were then intradermally injected evenly into the STSG-DSs. On 3, 4, 5, 7, and 10 d after creation of STSG-DS, skin sample sections were stained with hematoxylin and eosin to evaluate reepithelialization and angiogenesis. Results Treatment of STSG-DS with PRP&F-P M-NPs effectively promoted epithelialization and new vessel formation compared with those treated with PRP, F-P M-NPs, and control (saline). Conclusions The intradermal injection of PRP&F-P M-NPs promotes epithelialization and angiogenesis in STSG-DS wounds.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2014.08.011