Evaluation of repetitive element polymerase chain reaction for surveillance of methicillin-resistant Staphylococcus aureus at a large academic medical center and community hospitals

Abstract Repetitive element polymerase chain reaction (rep-PCR) typing has been used for methicillin-resistant Staphylococcus aureus (MRSA) strain characterization. The goal of this study was to determine if a rapid commercial rep-PCR system, DiversiLab™ (DL; bioMérieux, Durham, NC, USA), could be u...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2015, Vol.81 (1), p.13-17
Main Authors: Wang, Shu-Hua, Stevenson, Kurt B, Hines, Lisa, Mediavilla, José R, Khan, Yosef, Soni, Ruchi, Dutch, Wendy, Brandt, Eric, Bannerman, Tammy, Kreiswirth, Barry N, Pancholi, Preeti
Format: Article
Language:English
Subjects:
Academic Medical Centers
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Typing Techniques - methods
Electrophoresis, Gel, Pulsed-Field
Female
Hospitals, Community
Humans
Infection Control - methods
Infectious Disease
Internal Medicine
Male
Methicillin-resistant Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus - classification
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Middle Aged
Molecular Epidemiology - methods
molecular genotyping
MRSA
Ohio
PFGE
Polymerase Chain Reaction - methods
rep-PCR
Repetitive Sequences, Nucleic Acid
SCCmec
spa
Staphylococcal Infections - microbiology
Young Adult
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Summary:Abstract Repetitive element polymerase chain reaction (rep-PCR) typing has been used for methicillin-resistant Staphylococcus aureus (MRSA) strain characterization. The goal of this study was to determine if a rapid commercial rep-PCR system, DiversiLab™ (DL; bioMérieux, Durham, NC, USA), could be used for MRSA surveillance at a large medical center and community hospitals. A total of 1286 MRSA isolates genotyped by the DL system were distributed into 84 distinct rep-PCR patterns: 737/1286 (57%) were clustered into 6 major rep-PCR patterns. A subset of 220 isolates was further typed by pulsed-field gel electrophoresis (PFGE), spa typing, and SCC mec typing. The 220 isolates were distributed into 80 rep-PCR patterns, 94 PFGE pulsotypes, 27 spa , and 3 SCC mec types. The DL rep-PCR system is sufficient for surveillance, but the DL system alone cannot be used to compare data to other institutions until a standardized nomenclature is established and the DL MRSA reference library is expanded.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2014.05.005