The use of random amplified polymorphic DNA markers in wheat

An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic marker system in wheat. Reproducible amplification products were obtained from varietal, homozygous single chromosome recombinant line and wheat/alien addition line genomic DNA with selected primers and rigorou...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-08, Vol.84 (5-6), p.567-572
Main Authors: Devos, K.M. (Cambridge Laboratory, Norwich (United Kingdom)), Gale, M.D
Format: Article
Language:English
Subjects:
Biological and medical sciences
CARTE GENETIQUE
Classical genetics, quantitative genetics, hybrids
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
GENETIC MAPS
GENETIC MARKERS
Genetics of eukaryotes. Biological and molecular evolution
MAPAS GENETICOS
MARCADORES GENETICOS
Markers
MARQUEUR GENETIQUE
PCR
Pteridophyta, spermatophyta
RAPD
RESTRICTION ENZYMES
TRITICUM AESTIVUM
Vegetals
Wheat
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Summary:An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic marker system in wheat. Reproducible amplification products were obtained from varietal, homozygous single chromosome recombinant line and wheat/alien addition line genomic DNA with selected primers and rigorously optimized reaction conditions. Factors influencing the RAPD patterns are DNA concentration, Mg(2+) concentration, polymerase concentration and denaturing temperature. In wheat, the non-homoeologous, non-dose responsive and dominant behaviour of RAPD products devalues their use as genetic markers for the construction of linkage maps, and the high probability that the amplified fragments derive from repetitive DNA limits their use as a source of conventional RFLP probes. However, RAPD markers will most certainly find many applications in the analysis of genotypes where single chromosomes or chromosome segments are to be manipulated.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00224153