Effect of T3 hormone on neural differentiation of human adipose derived stem cells

Human adult stem cells, which are capable of self‐renewal and differentiation into other cell types, can be isolated from various tissues. There are no ethical and rejection problems as in the case of embryonic stem cells, so they are a promising source for cell therapy. The human body contains a gr...

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Bibliographic Details
Published in:Cell biochemistry and function 2014-12, Vol.32 (8), p.702-710
Main Authors: Razavi, Shahnaz, Mostafavi, Fatemeh Sadat, Mardani, Mohammad, Zarkesh Esfahani, Hamid, Kazemi, Mohammad, Esfandiari, Ebrahim
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adult
Biomarkers - metabolism
Cell Differentiation - drug effects
Cell Survival - drug effects
GFAP
hADSCs
Humans
MAP-2
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Middle Aged
neural induction
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Triiodothyronine - metabolism
Triiodothyronine - pharmacology
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Summary:Human adult stem cells, which are capable of self‐renewal and differentiation into other cell types, can be isolated from various tissues. There are no ethical and rejection problems as in the case of embryonic stem cells, so they are a promising source for cell therapy. The human body contains a great amount of adipose tissue that contains high numbers of mesenchymal stem cells. Human adipose‐derived stem cells (hADSCs) could be easily induced to form neuron‐like cells, and because of its availability and abundance, we can use it for clinical cell therapy. On the other hand, T3 hormone as a known neurotropic factor has important impressions on the nervous system. The aim of this study was to explore the effects of T3 treatment on neural differentiation of hADSCs. ADSCs were harvested from human adipose tissue, after neurosphere formation, and during final differentiation, treatment with T3 was performed. Immunocytochemistry, real‐time RT‐PCR, Western blotting techniques were used for detection of nestin, MAP2, and GFAP markers in order to confirm the effects of T3 on neural differentiation of hADSCs. Our results showed an increase in the number of glial cells but reduction in neuronal cells number fallowing T3 treatment. Copyright © 2014 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.3074