In utero exposure to perfluorooctanoate (PFOA) or perfluorooctane sulfonate (PFOS) did not increase body weight or intestinal tumorigenesis in multiple intestinal neoplasia (Min/+) mice

• Published in: Environmental research 2014-07, Vol.132, p.251-263
• Main Authors: Ngo, Ha Thi, Hetland, Ragna Bogen, Sabaredzovic, Azemira, Haug, Line Småstuen, Steffensen, Inger-Lise
• Format: Article
• Language: English
• Subjects: Age; Alkanesulfonic Acids - blood; Alkanesulfonic Acids - toxicity; Animals; Biological and medical sciences; Blood Glucose - drug effects; Body weight; Body Weight - drug effects; Caprylates - blood; Caprylates - toxicity; Carcinogenesis - drug effects; Carcinogenesis, carcinogens and anticarcinogens; Chemical agents; Dams; Eating - drug effects; Exposure; Female; Fluorocarbons - blood; Fluorocarbons - toxicity; Glucose; In utero exposure; Intestinal Neoplasms - chemically induced; Intestinal tumorigenesis; Intestines; Liver - drug effects; Male; Medical sciences; Mice; Mice, Inbred C57BL; Min/+ mouse; Obesity - chemically induced; Obesogen; Organ Size - drug effects; Perfluorooctane sulfonate; Perfluorooctanoate; Pregnancy; Reproduction - drug effects; Spleen - drug effects; Sulfonates; Tumors
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2014.03.033

We examined whether perfluorooctanoate (PFOA) or perfluorooctane sulfonate (PFOS) had obesogenic effects and if they increased spontaneous intestinal tumorigenesis in the mouse model C57BL/6J-Min/+ (multiple intestinal neoplasia) after in utero exposure. The dams were exposed to PFOA or PFOS (0.01, 0.1 or 3.0mg/kg bw/day) by po gavage on GD1–17. The Min/+ and wild-type offspring were terminated at week 11 for examination of intestinal tumorigenesis or at week 20 for obesogenic effect, respectively. Body weights of the dams and pups were recorded throughout life. Food intake was determined at week 6 and 10. Blood glucose (non-fasted) was measured at week 6 and 11. No obesogenic effect of PFOA or PFOS was observed up to 20 weeks of age. PFOA or PFOS did not increase the incidence or number of tumors in the small intestine or colon of the Min/+ mice or affect their location along the intestines. Feed intake was not affected. There were some indications of toxicity of PFOA, but not of PFOS. There was lower survival of pups after 3.0mg/kg PFOA, lower body weight in pups after 3.0 and possibly 0.1mg/kg PFOA, and increased relative liver weight after 0.01 and possibly 0.1mg/kg PFOA. Plasma glucose was lower after 0.01 and 0.1mg/kg PFOA. In conclusion, exposure to PFOA and PFOS in utero with the doses used did not have obesogenic effect on either Min/+ or wild-type mice, at least not up to 11 or 20 weeks of age, nor increased intestinal tumorigenesis in Min/+ mice. •Perfluorooctanoate (PFOA) had no obesogenic effect in mice up to age 20 weeks.•Perfluorooctane sulfonate (PFOS) had no obesogenic effect in mice up to age 20 weeks.•PFOA did not increase intestinal tumorigenesis in Min/+ mice.•PFOS did not increase intestinal tumorigenesis in Min/+ mice.•Some indications of toxicity of PFOA, but not PFOS, were found.