Facile detection of photogenerated reactive oxygen species in TiO2 nanoparticles suspension using colorimetric probe-assisted spectrometric method

•We measured photogenerated ROS in TiO2 NPs suspension with spectrometric method.•TiO2 NPs were selectively separated from suspension by centrifugation.•The high ionic strength ([KH2PO4]0=50mM) was required to selective separation.•The ROS was monitored by UV–vis Spectroscopy without interfering eff...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2013-11, Vol.93 (9), p.2011-2015
Main Authors: Kim, Choonsoo, Park, Hee-jin, Cha, Seoeun, Yoon, Jeyong
Format: Article
Language:English
Subjects:
Applied sciences
Buffers
centrifugation
Colorimetry
Exact sciences and technology
Global environmental pollution
Hydroxyl Radical - chemistry
Interference
ionic strength
Metal Nanoparticles - analysis
Metal Nanoparticles - chemistry
Models, Chemical
Nanoparticle interference
Nanoparticles
oxygen
Phosphates
Photochemical Processes
Pollution
Radicals
reactive oxygen species
Reactive Oxygen Species - chemistry
Selective seperation
Spectrophotometric ROS detection
Spectroscopy
Superoxides - chemistry
tetrazolium
TiO2 nanoparticles suspension
Titanium - analysis
Titanium - chemistry
Titanium dioxide
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Summary:•We measured photogenerated ROS in TiO2 NPs suspension with spectrometric method.•TiO2 NPs were selectively separated from suspension by centrifugation.•The high ionic strength ([KH2PO4]0=50mM) was required to selective separation.•The ROS was monitored by UV–vis Spectroscopy without interfering effect of the NPs. This present study reports that reactive oxygen species (ROS), which mainly consist of hydroxyl (OH) and superoxide (O2-) radicals generated by the photocatalysis of TiO2 nanoparticles (TiO2 NPs) in suspension, can be quantitatively measured by the colorimetric probe-assisted spectrometric method without any interference by the TiO2 NPs. The interference effect of the TiO2 NPs with UV–vis spectroscopy was excluded by the selective separation of p-nitrosodimethylaniline (RNO) and tetrazolium salt (XTT) (probe compounds for OH and O2-, respectively) from the TiO2 NPs in the suspension by centrifugation at a specified ionic strength adjusted with phosphate buffer (KH2PO4/NaOH, PB) solution. This colorimetric probe-assisted spectrometric method was also successfully applied to measure the steady-state production of photogenerated ROS in a TiO2 NPs suspension.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2013.07.023