Spongy graphene electrode in electrochemical detection of leukemia at single-cell levels

Mg2+-charged spongy graphene electrodes (SGEs) were fabricated by using electrophoretic deposition of chemically exfoliated graphene oxide sheets on graphite rods. The SGEs were able to present two distinguishable signals (originated from electrochemical oxidation of guanine) in differential pulse v...

Full description

Saved in:
Bibliographic Details
Published in:Carbon (New York) 2014-11, Vol.79, p.654-663
Main Authors: Akhavan, Omid, Ghaderi, Elham, Rahighi, Reza, Abdolahad, Mohammad
Format: Article
Language:English
Subjects:
Blood cells
Carbon
Chemistry
Cross-disciplinary physics: materials science
rheology
Electrochemistry
Electrodeposition, electroplating
Electrodes
Exact sciences and technology
General and physical chemistry
Glassy carbon
Graphene
Leukemias
Materials science
Methods of deposition of films and coatings
film growth and epitaxy
Methods of nanofabrication
Physics
Rods
Voltammetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mg2+-charged spongy graphene electrodes (SGEs) were fabricated by using electrophoretic deposition of chemically exfoliated graphene oxide sheets on graphite rods. The SGEs were able to present two distinguishable signals (originated from electrochemical oxidation of guanine) in differential pulse voltammetry (DPV) of leukemia and normal blood cells, in contrast to glassy carbon electrodes giving only one overlapped peak. Hence, the SGEs were applied in fast (60min) and ultra sensitive detection of leukemia (single abnormal cells in ∼109 normal cells) in a blood serum. The sensitivity obtained by the SGEs was three orders of magnitude better than that of the best available and current technologies (e.g., specific mutations by polymerase chain reaction with detection limit of one abnormal cell in ∼106 normal cells) which not only are expensive, but also require several days for incubation. Significant variations in DPV signals of the SGEs after the first electrochemical cycle indicated that the best performance of the SGEs can be achieved only at the first cycle. The linear dynamic detection behavior of the SGEs was investigated in wide concentration range of 1.0×105–0.1cell/mL. The lower detection limit was estimated ∼0.02cell/mL, based on the current resolution obtained by the SGEs.
ISSN:0008-6223
1873-3891
DOI:10.1016/j.carbon.2014.08.058