Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles

We report on a lateral flow immunoassay (LFIA) for influenza A antigen using fluorescently-doped silica nanoparticles as reporters. The method is taking advantage of the high brightness and photostability of silica nanoparticles (doped with the dye Cy5) and the simplicity and rapidity of LFIA. The n...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2014-01, Vol.181 (1-2), p.223-230
Main Authors: Bamrungsap, Suwussa, Apiwat, Chayachon, Chantima, Warangkana, Dharakul, Tararaj, Wiriyachaiporn, Natpapas
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antigens
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Computational fluid dynamics
Ethylenediaminetetraacetic acid
Fluorescence
Immunoassay
Influenza
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Povidone
Proteins
Recombinant
Silica
Silicon dioxide
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Summary:We report on a lateral flow immunoassay (LFIA) for influenza A antigen using fluorescently-doped silica nanoparticles as reporters. The method is taking advantage of the high brightness and photostability of silica nanoparticles (doped with the dye Cy5) and the simplicity and rapidity of LFIA. The nucleoprotein of influenza A virion (one of its most abundant structural proteins) was used as a model to demonstrate a performance of the LFIA. Under optimized conditions and by using a portable strip reader, the fluorescence-based LFIA is capable of detecting a recombinant nucleoprotein as low as 250 ng · mL -1 using a sample volume of 100 μL, within 30 min, and without interference by other proteins. The successful detection of the nucleoprotein in infected allantoic fluid demonstrated the functionality of the method. By comparison with a commercial influenza A test based on gold nanoparticles as reporters, the system provides an 8-fold better sensitivity. Figure A rapid and sensitive lateral flow immunoassay for influenza A antigen was developed using fluorescently-doped silica nanoparticles. A sample containing nucleoprotein as a target analyte induced an accumulation of the fluorescent conjugates at the test spot. The signal was then measured quantitatively using a portable strip reader.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-013-1106-4