Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure

• Published in: Bioresource technology 2013-12, Vol.150, p.377-386
• Main Authors: Kobayashi, Naoko, Noel, Eric A., Barnes, Austin, Watson, Andrea, Rosenberg, Julian N., Erickson, Galen, Oyler, George A.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Ammonium Compounds - metabolism; Anaerobic digester effluent; Anaerobiosis; Animals; Ashes; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Biomass; Biotechnology; Cattle; Chlorella; Chlorella - drug effects; Chlorella - growth & development; Chlorella sorokiniana; Culture Media - pharmacology; Effluents; Environment and pollution; Esters - analysis; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Industrial applications and implications. Economical aspects; Lipids; Manure; Nitrates - metabolism; Nitrites - metabolism; Nitrogen - metabolism; Nutrient removal; Phosphorus - metabolism; Starch; Starch - metabolism; Starches; Use of agricultural and forest wastes. Biomass use, bioconversion; Waste Disposal, Fluid
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.10.032

•Chlorella strains were compared in cattle anaerobic digested effluent and Bold’s Basel Media.•Algae biomass production is dependent on nutrient provision and removal from growth media.•Starch and protein production was greater than lipid accumulation in anaerobic digested effluent.•Chlorella grown in anaerobic digested effluent may be more suitable for animal feed application. Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold’s Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280mg/L after 21days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty acid methyl ester total lipid measured less than 12% AFDW. Results suggest that biomass from UTEX 1230 in ADE may serve as a candidate alga and growth system combination sustainable for animal feed production considering high yields of protein, starch and low lipid accumulation.