Effect of feeding behavior on circadian regulation of endothelin expression in mouse colon

The function, regulation and gene expression of the endothelin (ET) system in the intestine is not well understood. We investigated the dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon. Mice were fed freely, fasted for 48h and re-fed after...

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Bibliographic Details
Published in:Life sciences (1973) 2014-11, Vol.118 (2), p.232-237
Main Authors: Kozakai, Takaharu, Sakate, Mitsue, Takizawa, Satoshi, Uchide, Tsuyoshi, Kobayashi, Hisato, Oishi, Katsutaka, Ishida, Norio, Saida, Kaname
Format: Article
Language:English
Subjects:
Animals
Autonomic Nervous System - metabolism
Circadian
Circadian Rhythm - genetics
Colon - innervation
Colon - metabolism
Endothelin
Endothelin-1 - genetics
Endothelin-1 - metabolism
Endothelin-2 - genetics
Endothelin-2 - metabolism
Expression
Fasting
Feeding Behavior
Gastrointestinal hormones
Gene Expression Regulation
Male
Mice, Inbred ICR
Peptides - genetics
Peptides - metabolism
Period Circadian Proteins - genetics
Period Circadian Proteins - metabolism
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Summary:The function, regulation and gene expression of the endothelin (ET) system in the intestine is not well understood. We investigated the dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon. Mice were fed freely, fasted for 48h and re-fed after fasting. Where indicated ET-1 gene expression was highest in the colon compared with other tissues examined in fasted mice. Fasting increased the level, while maintaining the rhythmicity, of ET-1 gene expression in epithelial colonic tissue. Re-feeding, however, decreased ET-1 gene expression and suppressed rhythmic oscillation, and the rhythmicity also changed for gene expression for circadian clocks, period-1 and period-2 (Per1 and Per2). Furthermore, the decrease in ET-1 gene expression induced by re-feeding was blocked by pre-treatment with hexamethonium and atropine. The daily change in ET-1 gene expression in colon, which depends on feeding schedule via the autonomic nervous system, is synchronized with peripheral circadian oscillators under conditions of free feeding and fasting but not re-feeding. The decrease in ET-1 gene expression in the proximal colon induced by re-feeding occurs via the nervous system. ET-1 plays an important physiological role, which is dependent on feeding behavior. [Display omitted]
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2014.06.022