Impact of aging on spreading depolarizations induced by focal brain ischemia in rats

Abstract Spreading depolarization (SD) contributes to the ischemic damage of the penumbra. Although age is the largest predictor of stroke, no studies have examined age dependence of SD appearance. We characterized the electrophysiological and hemodynamic changes in young (6 weeks old, n  = 7), midd...

Full description

Saved in:
Bibliographic Details
Published in:Neurobiology of aging 2014-12, Vol.35 (12), p.2803-2811
Main Authors: Clark, Darren, Institoris, Ádám, Kozák, Gábor, Bere, Zsófia, Tuor, Ursula, Farkas, Eszter, Bari, Ferenc
Format: Article
Language:English
Subjects:
Aging - pathology
Aging - physiology
Animals
Brain Ischemia - pathology
Brain Ischemia - physiopathology
Cerebral Cortex - blood supply
Cerebral Cortex - pathology
Cerebrovascular Circulation
Electrophysiological Phenomena
Hemodynamics
Internal Medicine
Inverse neurovascular coupling
Male
Membrane Potentials
Middle cerebral artery occlusion
Multimodal imaging
Neurology
Peri-infarct depolarization
Rats, Wistar
Voltage-sensitive dye
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Spreading depolarization (SD) contributes to the ischemic damage of the penumbra. Although age is the largest predictor of stroke, no studies have examined age dependence of SD appearance. We characterized the electrophysiological and hemodynamic changes in young (6 weeks old, n  = 7), middle-aged (9 months old, n  = 6), and old (2 years old, n  = 7) male Wistar rats during 30 minutes of middle cerebral artery occlusion (MCAO), utilizing multimodal imaging through a closed cranial window over the ischemic cortex: membrane potential changes (with a voltage-sensitive dye), cerebral blood volume (green light reflectance), and cerebral blood flow (CBF, laser-speckle imaging) were observed. The initial CBF drop was similar in all groups, with a significant further reduction during ischemia in old rats ( p < 0.01). Age reduced the total number of SDs ( p < 0.05) but increased the size of ischemic area displaying prolonged SD ( p < 0.01). The growth of area undergoing prolonged SDs positively correlated with the growth of ischemic core area ( p < 0.01) during MCAO. Prolonged SDs and associated hypoperfusion likely compromise cortical tissue exposed to even a short focal ischemia in aged rats.
ISSN:0197-4580
1558-1497
DOI:10.1016/j.neurobiolaging.2014.06.013