Structural studies of virus–antibody immune complexes (poliovirus type I): Characterization of the epitopes in 3D

• Published in: Molecular immunology 2015-02, Vol.63 (2), p.279-286
• Main Authors: Bannwarth, Ludovic, Girerd-Chambaz, Yves, Arteni, Ana A., Guigner, Jean-Michel, Lemains, Jacques, Ronzon, Frédéric, Manin, Catherine, Vénien-Bryan, Catherine
• Format: Article
• Language: English
• Subjects: Amino Acids - chemistry; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; Antigen-Antibody Complex - chemistry; Antigen-Antibody Complex - immunology; Antigenic site; Antigens, Viral - chemistry; Antigens, Viral - immunology; Cryo-electron microscopy; Cryoelectron Microscopy; Crystallography, X-Ray; Epitopes - chemistry; Epitopes - immunology; Image analysis; Imaging, Three-Dimensional; Immunoglobulin Fab Fragments - chemistry; Immunoglobulin Fab Fragments - immunology; Models, Molecular; Monoclonal antibody; Polio vaccine; Poliovirus - chemistry; Poliovirus - immunology; Poliovirus - ultrastructure; Poliovirus type 1; Protein Footprinting
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2014.07.014

•TEM analysis of poliovirus–Fab and poliovirus–Mab complexes.•Description of the epitopes recognized by anti-type 1 poliovirus Fabs 234, 236, 237, 423 and 73-5-5.•The antigenic site for Mab73-5-5 is located at the bottom of the canyon. The inactivated polio vaccine (IPV) contains poliovirus (PVs) samples that belong to serotypes 1, 2 and 3. All three serotypes contain the D-antigen, which induces protective antibodies. The antigenic structure of PVs consists of at least four different antigenic sites and the D-antigen content represents the combined activity of multiple epitopes (Ferguson et al., 1993; Minor, 1990; Minor et al., 1986). The potency of IPV vaccines is determined by measuring the D-antigen content. Several ELISA methods have been developed using polyclonal or monoclonal antibodies (Mabs) in order to quantify the D-antigen content. Characterization of the epitopes recognized by the different Mabs is crucial to map the entire virus surface and ensure the presence of epitopes able to induce neutralizing antibodies. In a new approach, combining cryo-electron microscopy and image analysis with X-ray crystallography data available along with identification of exposed amino acids we have mapped in 3D the epitope sites recognized by five specific Fabs and one Mab and characterized precisely the antigenic sites for these Mabs. We propose this method to be used to map the entire “epitopic” surface of virus.