Coke oven workers study: the effect of exposure and GSTM1 and NAT2 genotypes on DNA adduct levels in white blood cells and lymphocytes as determined by super(32)P-postlabelling

The DNA adduct levels in total white blood cells (WBC) and lymphocytes (LYM) isolated from the blood of the same individuals were evaluated using the super(32) P -postlabelling assay for bulky aromatic adducts. In this study, 68 male coke oven workers and 56 machine workers as a matched control were...

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Published in:Mutation research. Genetic toxicology and environmental mutagenesis 1998-08, Vol.416 (1-2), p.67-84
Main Authors: Binkova, F, Topinka, J, Mrackova, G, Gajdosova, D, Vidova, P, Stavkova, Z, Peterka, V, Pilcik, T, Rimar, V, Dobias, L, Farmer, P B, Sram, R J
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Language:English
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Summary:The DNA adduct levels in total white blood cells (WBC) and lymphocytes (LYM) isolated from the blood of the same individuals were evaluated using the super(32) P -postlabelling assay for bulky aromatic adducts. In this study, 68 male coke oven workers and 56 machine workers as a matched control were enrolled. Personal monitors were used to evaluate exposure to eight carcinogenic PAHs, including B[a]P, during an 8-h working shift. The exposure among coke oven workers ranged widely from 0.6 to 547 mu g /m super(3) and from 2 to 62,107 ng/m super(3), for carcinogenic PAHs and B[a]P, respectively. The respective values in controls were from 0.07-1.64 mu g/m super(3) and from 1-63 ng/m super(3). A significant correlation between WBC- and LYM-DNA adduct levels was found (r=0.591, P
ISSN:1383-5718