Combination of resistance to potato late blight in foliage and tubers by intraspecific dihaploid protoplast fusion

Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybr...

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Bibliographic Details
Published in:Euphytica 1998-01, Vol.102 (3), p.363-370
Main Authors: Rasmussen, J.O. (Aarhus Univ. (Denmark). Dept. of Molecular and Structural Biology), Nepper, J.P, Kirk, H.G, Tolstrup, K, Rasmussen, O.S
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
DIHAPLOIDIA
DIHAPLOIDIE
DIHAPLOIDY
DISEASE RESISTANCE
Flowers & plants
Foliage
Fundamental and applied biological sciences. Psychology
GENETIC RESISTANCE
Genetics and breeding of economic plants
Genotypes
HIBRIDOS SOMATICOS
HYBRIDE SOMATIQUE
Hybrids
Laboratory tests
Pest resistance
PHYTOPHTHORA INFESTANS
Plant pathogens
Potatoes
RAPD
RESISTANCE AUX MALADIES
RESISTANCE GENETIQUE
RESISTENCIA A LA ENFERMEDAD
RESISTENCIA GENETICA
SOLANUM TUBEROSUM
SOMATIC HYBRIDS
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybrid calli were selected four weeks after electrofusion. Intact plants were regenerated from 6% of the selected calli. Verification of hybridity was accomplished by use of RAPD analysis which revealed that 53% of the regenerated plants were true somatic hybrids. The score of true somatic hybrids in the different fusion combinations ranged within 21% - 100%. The hybrid plants were analysed for resistance to foliage blight in a field trial and assessed for tuber blight resistance by use of a laboratory test. Resistance to late blight in foliage and tubers varied between the hybrids. Very high levels of resistance to both foliage and tuber blight were obtained in some hybrids. However, loss of resistance in some hybrids as compared to the parental plants were also observed. Possible reasons for the phenotypic disappearance of resistance to either foliage or tuber blight are suggested.[PUBLICATION ABSTRACT]
ISSN:0014-2336
1573-5060
DOI:10.1023/A:1018302103100