Determination of drugs of abuse in bovine dentin using liquid chromatography-electrospray ionization tandem mass spectrometry

Drugs deposited in human teeth are well preserved; the spectrum of toxicological investigations may therefore be supplemented by an analysis method for drugs in teeth. A liquid chromatography–electrospray ionization tandem mass spectrometry assay for the detection and quantification of basic drugs o...

Full description

Saved in:
Bibliographic Details
Published in:Journal of mass spectrometry. 2014-12, Vol.49 (12), p.1306-1313
Main Authors: Spinner, J., Klima, M., Kempf, J., Huppertz, L. M., Auwärter, V., Altenburger, M. J., Neukamm, M. A.
Format: Article
Language:English
Subjects:
alternative matrix
Animals
Cattle
Chromatography
Chromatography, Liquid - methods
dental hard tissue
Dentin
Dentin - chemistry
Drugs
drugs of abuse
Humans
Ionization
LC-ESI-MS/MS
Limit of Detection
Liquids
Mass spectrometry
Methyl alcohol
Spectrometry, Mass, Electrospray Ionization - methods
Street Drugs - analysis
Substance Abuse Detection - methods
Tandem Mass Spectrometry - methods
Teeth
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Drugs deposited in human teeth are well preserved; the spectrum of toxicological investigations may therefore be supplemented by an analysis method for drugs in teeth. A liquid chromatography–electrospray ionization tandem mass spectrometry assay for the detection and quantification of basic drugs of abuse in bovine dentin samples was developed and validated. The drugs and metabolites amphetamine, methamphetamine, methylenedioxymethylamphetamine, methylenedioxyethylamphetamine, codeine, morphine, cocaine and benzoylecgonine were extracted from 50 mg ground dentin powder by ultrasonication for 60 min in methanol 3 times. The extracts were analyzed on a triple‐quadrupole mass‐spectrometer in multiple reaction monitoring mode. The method was validated and proved to be accurate, precise, selective, specific and stable with good linearity within the calibration range and a lower limit of quantification of 10 to 20 pg/mg. To artificially load bovine dentin samples with drugs, the natural process of de‐ and remineralization in the oral cavity was mimicked by a pH‐cycling experiment. The artificially drug‐loaded dentin samples showed drug concentrations of 20 to 80 pg/mg. The method can be applied in further in vitro experiments as well as in post‐mortem cases, especially where limited sample tissue is available. Copyright © 2014 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.3464