A novel electrospun-aligned nanoyarn-reinforced nanofibrous scaffold for tendon tissue engineering

•A novel SF/P(LLA-CL) aligned nanoyarn-reinforced nanofibrous scaffold was prepared using a modified electrospinning method.•Tensile strength of the novel scaffold was improved by the aligned nanoyarns.•The novel nanoyarn scaffold provides higher porosity and bigger pore size for MSCs infiltration t...

Full description

Saved in:
Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-10, Vol.122, p.270-276
Main Authors: Yang, Chengwei, Deng, Guoying, Chen, Weiming, Ye, Xiaojian, Mo, Xiumei
Format: Article
Language:English
Subjects:
Alignment
Animals
Biocompatible Materials
Cell infiltration
Cell Proliferation
Electrospinning
Infiltration
Mesenchymal Stromal Cells - cytology
Nanostructure
Nanostructures
Nanoyarn
Porosity
Rats
Rats, Sprague-Dawley
Scaffolds
Tendon tissue engineering
Tendons
Tendons - growth & development
Three-dimensional scaffold
Tissue Engineering
Tissue Scaffolds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•A novel SF/P(LLA-CL) aligned nanoyarn-reinforced nanofibrous scaffold was prepared using a modified electrospinning method.•Tensile strength of the novel scaffold was improved by the aligned nanoyarns.•The novel nanoyarn scaffold provides higher porosity and bigger pore size for MSCs infiltration three dimensionally.•The novel nanoyarn scaffold meets the mechanical and biological requirements for tendon tissue engineering. An electrospun-aligned nanoyarn-reinforced nanofibrous scaffold (NRS) was developed for tendon tissue engineering to improve mechanical strength and cell infiltration. The novel scaffold composed of aligned nanoyarns and random nanofibers was fabricated via electrospinning using a two-collector system. The aim of the present study was to investigate three different types of electrospun scaffolds (random nanofibrous scaffold, aligned nanofibrous scaffold and NRS) based on silk fibroin (SF) and poly(l-lactide-co-caprolactone) blends. Morphological analysis demonstrated that the NRS composed of aligned nanoyarns and randomly distributed nanofibers formed a 3D microstructure with relatively large pore sizes and high porosity. Biocompatibility analysis revealed that bone marrow-derived mesenchymal stem cells exhibited a higher proliferation rate when cultured on the NRS compared with the other scaffolds. The mechanical testing results indicated that the tensile properties of the NRS were reinforced in the direction parallel to the nanoyarns and satisfied the mechanical requirements for tendon repair. In addition, cell infiltration was significantly enhanced on the NRS. In conclusion, with its improved porosity and appropriate mechanical properties, the developed NRS shows promise for tendon tissue engineering applications.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2014.06.061