Anti-inflammatory and anti-bacterial activity, and CYTOTOXICITY of halloysite surfaces

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-11, Vol.111, p.651-655
• Main Authors: Cervini-Silva, Javiera, Nieto-Camacho, Antonio, Palacios, Eduardo, Montoya, José Ascención, Gómez-Vidales, Virginia, Ramírez-Apán, María Teresa
• Format: Article
• Language: English
• Subjects: acidity; Aluminum Silicates - toxicity; Animals; Anti-Bacterial Agents - pharmacology; anti-inflammatory activity; anti-inflammatory agents; Anti-Inflammatory Agents - pharmacology; antibacterial properties; antioxidant activity; biomedical research; biotechnology; Cell Death - drug effects; Cell Survival - drug effects; cell viability; colloids; Constraining; cytotoxicity; ears; edema; Electrons; enzyme activity; halloysite; Infiltration; Invariants; Ion Exchange; Macrophages, Peritoneal - cytology; Macrophages, Peritoneal - drug effects; Magnetic Resonance Spectroscopy; Mice; myeloperoxidase; nanomaterials; Nanostructure; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Order disorder; Peroxidase - metabolism; protons; surface area; Surface chemistry; Surface morphology; Surface Plasmon Resonance; Surface Properties; Tetradecanoylphorbol Acetate; Time-dependent infiltration; Viability
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2013.06.056

The surface properties of halloysite control its anti-inflammatory and anti-oxidant activity, and cytoxicity properties. •Surface properties determine the anti-inflammatory activity of halloysite.•The long-term anti-inflammatory activity is explained by an edge-controlled mechanism.•Occlusions and decreases in Aloct account for by a poor stabilization of RO species.•Cell viability depends on surface morphology and relates to surface area. Halloysite is a naturally-occurring nanomaterial occurring in the thousands of tons and that serves as biomaterial, with applications in the areas of biotechnology, pharmaceutical, and medical research. This study reports on the anti-inflammatory, cytotoxic, and anti-oxidant activity of halloysite Jarrahdale (collected at ∼45km SE of Perth, Western Australia; JA), Dragon Mine (provided by Natural Nano Inc., Rochester, New York; NA), and Kalgoorie Archean (collected at Siberia, ∼85km NW of Kalgoorlie, West Australia; PA). Prior to biological testing, halloysites were characterized by 27Al and 29Si Nuclear Magnetic Resonance Spectroscopy, the anti-inflammatory activity was determined by (a) the mouse ear edema method, using 12-o-tetradecanoylphorbol-13-acetate (TPA) as anti-inflammatory agent; and (b) the myeloperoxidase enzymatic activity method (MPO). Cell viability was determined using the MTT method. Sample characterization by NMR method showed similar symmetry and atomic environments, with no evidence of distortion(s) due to shiftings in atomic ordering or electron density. The anti-inflammatory activity followed the order: PA>JA>NA, and remained invariant with time. Prolonged anti-inflammatory activity related inversely to surface area and lumen space. The low extent of infiltration at shorter reaction times confirmed a limiting number of active surface sites. EPR intensity signals followed the order: JA>NA>PA. The poor stabilization of RO species in PA suspensions was explained by tube alignment provoking occlusion, thus limiting transfer of H+ or e− from-and-to the surface, and decreases in acidity associated to Aloct. Cell viability (%) varied from one surface to the other, PA(92.3±6.0), JA(84.9±7.8), and NA(78.0±5.6), but related directly to SBET values.