The effect of lightly crosslinked poly(carboxybetaine) hydrogel coating on the performance of sensors in whole blood

Abstract Surface coatings of high packing densities have been routinely used to prevent nonspecific biomolecular and microorganism attachment. Hydrogels are another class of low fouling materials used to create three-dimensional matrixes for the free diffusion of small analytes or drugs and the high...

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Bibliographic Details
Published in:Biomaterials 2012-11, Vol.33 (32), p.7945-7951
Main Authors: Yang, Wei, Bai, Tao, Carr, Louisa R, Keefe, Andrew J, Xu, Jiajie, Xue, Hong, Irvin, Colleen A, Chen, Shengfu, Wang, Joseph, Jiang, Shaoyi
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Betaine - analogs & derivatives
Biosensing Techniques - methods
Biosensor
Blood
Blood Glucose - analysis
Blood Glucose - metabolism
Cercopithecus aethiops
Coated Materials, Biocompatible - chemistry
Coatings
COS Cells
Cross-Linking Reagents - chemistry
Crosslinking
Dentistry
Diffusion
Enzymes, Immobilized - metabolism
Glucose
Glucose Oxidase - metabolism
Humans
Hydrogel
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogels
Packing density
Polymerization
Polymers - chemistry
Sensitivity and Specificity
Sensors
Three dimensional
Whole blood
Zwitterionic
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Summary:Abstract Surface coatings of high packing densities have been routinely used to prevent nonspecific biomolecular and microorganism attachment. Hydrogels are another class of low fouling materials used to create three-dimensional matrixes for the free diffusion of small analytes or drugs and the high-loading of bio-recognition elements. However, biomolecules are subject to being entrapped within hydrogel matrixes or adhered onto hydrogel surfaces, making them questionable for use in whole blood. Here, we demonstrate the feasibility of a lightly crosslinked poly(carboxybetaine) hydrogel for use in whole blood, as opposite to the conventional wisdom of high packing density in surface coatings. Proteins are able to diffuse in and out of the matrix freely without being altered from their native conformations. In order to demonstrate its long-term performance in whole blood, this hydrogel was used as the surface coating of a glucose sensor. This work paves a new way for the development of surface coatings and sensors to achieve long-term stability and high performance in whole blood.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.07.035