Chemically-induced redox switching of a metalloprotein reveals thermodynamic and kinetic heterogeneity, one molecule at a time

Oxidation (off state) and reduction (on state) of a single azurin molecule is monitored, one electron at a time, which depend on the chemical redox potential. By analysing the fluorescence time traces from individual azurin molecules, reaction kinetics and redox thermodynamics were determined.

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2014-01, Vol.50 (93), p.14523-14526
Main Authors: Akkilic, Namik, van der Grient, Fenna, Kamran, Muhammad, Sanghamitra, Nusrat J M
Format: Article
Language:English
Subjects:
Azurin - chemistry
Electrode potentials
Electrons
Fluorescence
Heterogeneity
Kinetics
Oxidation
Oxidation-Reduction
Reaction kinetics
Reduction
Switching
Thermodynamics
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxidation (off state) and reduction (on state) of a single azurin molecule is monitored, one electron at a time, which depend on the chemical redox potential. By analysing the fluorescence time traces from individual azurin molecules, reaction kinetics and redox thermodynamics were determined.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc06334a