HNA-3 gene frequencies in Brazilians and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-3a/3b genotyping

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2014-06, Vol.54 (6), p.1619-1621
• Main Authors: Lopes, Larissa Barbosa, Baleotti Jr, Wilson, Suzuki, Rodrigo Buzinaro, Fabron Jr, Antonio, Chiba, Akemi Kuroda, Vieira-Filho, João Paulo Botelho, de Souza Castro, Bianca, Midori Kunioshi, Alessandra, Bordin, José Orlando
• Format: Article
• Language: English
• Subjects: Alleles; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Asian Continental Ancestry Group - genetics; Biological and medical sciences; Blood transfusions; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; European Continental Ancestry Group - genetics; Gene Frequency - genetics; Genes; Genotype; Humans; Indians, North American - genetics; Isoantigens - genetics; Medical sciences; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1111/trf.12493

Background HNA‐3 antigens are the result of a rs2288904 single‐nucleotide polymorphism (SNP) in the CTL2, and the HNA‐3a and HNA‐3b variants are encoded by a guanine and adenine at Nucleotide Position 461. Anti‐HNA‐3 are involved in severe transfusion‐related acute lung injury reactions and in neonatal alloimmune neutropenia. Since the distribution of the HNA‐3 system was unknown in South Americans, in this study we determined the frequency of the HNA‐3 alleles in Brazilians. Study Design and Methods DNA of 500 blood donors, 120 Xikrin Amerindians, 74 Japanese individuals, and 124 African Brazilians were genotyped for rs2288904 by a polymerase chain reaction (PCR)‐restriction fragment length polymorphism assay. The PCR product was digested with enzyme Taqα1, specific to nucleotide guanine (HNA‐3a). Results The results showed that the frequencies of the HNA‐3a/HNA‐3b alleles were 0.81/0.19 in blood donors, 1.00/0.00 in Amerindians, 0.63/0.37 in Japanese, and 0.85/0.15 in African Brazilians. All 81 individuals genotyped as HNA‐3a/a did not present the SNP c.457T by molecular sequencing. Conclusion The frequencies of HNA‐3 genotypes in Brazilian blood donors is similar to that described in Caucasians; however, all Amerindians were HNA‐3a/a, African Brazilians showed a lower frequency of HNA‐3b/b, and Japanese had a higher prevalence of HNA‐3b/b, suggesting that they may be at risk for developing anti‐HNA‐3a alloantibodies.