Virus–host interactions in persistently FMDV-infected cells derived from bovine pharynx

Abstract Foot-and-mouth disease virus (FMDV) produces a disease in cattle characterized by vesicular lesions and a persistent infection with asymptomatic low-level production of virus in pharyngeal tissues. Here we describe the establishment of a persistently infected primary cell culture derived fr...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2014-11, Vol.468, p.185-196
Main Authors: O’Donnell, V, Pacheco, J.M, Larocco, Michael, Gladue, D.P, Pauszek, S.J, Smoliga, G, Krug, P.W, Baxt, B, Borca, M.V, Rodriguez, L
Format: Article
Language:English
Subjects:
Animals
Bovine pharynx
Cattle
Cells
Cytokines - genetics
Cytokines - metabolism
FMDV
Foot-and-mouth disease virus
Foot-and-Mouth Disease Virus - physiology
Gene Expression Regulation
Host-Pathogen Interactions
Infectious Disease
Persistence
Pharynx - cytology
Picornavirus
Time Factors
Virus Replication
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Summary:Abstract Foot-and-mouth disease virus (FMDV) produces a disease in cattle characterized by vesicular lesions and a persistent infection with asymptomatic low-level production of virus in pharyngeal tissues. Here we describe the establishment of a persistently infected primary cell culture derived from bovine pharynx tissue (PBPT) infected with FMDV serotype O1 Manisa, where surviving cells were serially passed until a persistently infected culture was generated. Characterization of the persistent virus demonstrated changes in its plaque size, ability to grow in different cell lines, and change in the use of integrins as receptors, when compared with the parental virus. These results demonstrate the establishment of persistently infected PBPT cell cultures where co-adaptation has taken place between the virus and host cells. This in vitro model for FMDV persistence may help further understanding of the molecular mechanisms of the cattle carrier state.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2014.08.004