Formyl peptide receptor chimeras define domains involved in ligand binding

• Published in: The Journal of biological chemistry 1993-02, Vol.268 (4), p.2292-2295
• Main Authors: PEREZ, H. D, HOLMES, R, VILANDER, L. R, ADAMS, R. R, MANZANA, W, JOLLEY, D, ANDREWS, W. H
• Format: Article
• Language: English
• Subjects: Base Sequence; Biological and medical sciences; C5a antigen; Cell receptors; Cell structures and functions; domains; formyl peptides; Fundamental and applied biological sciences. Psychology; fusion protein; In Vitro Techniques; leukocytes (neutrophilic); ligand binding; man; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Miscellaneous; Models, Molecular; Molecular and cellular biology; Molecular Sequence Data; Mutagenesis, Site-Directed; N-Formylmethionine Leucyl-Phenylalanine - metabolism; Receptor, Anaphylatoxin C5a; receptors; Receptors, Complement - chemistry; Receptors, Formyl Peptide; Receptors, Immunologic - chemistry; Receptors, Immunologic - metabolism; Recombinant Fusion Proteins - chemistry; Structure-Activity Relationship
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)53771-6

We have begun to study the structural requirements for the binding of formyl peptides to their specific receptors. As an initial approach, we constructed C5a-formyl peptide receptor chimeras. Unique (and identical) restriction sites were introduced within the transmembrane domains of these receptors that allowed for the exchange of specific areas. Four types of chimeric receptors were generated. 1) The C5a receptor was progressively substituted by the formyl peptide receptor. 2) The formyl peptide receptor was progressively substituted by the C5a receptor. 3) Specific domains of the C5a receptor were substituted by the corresponding domain of the formyl peptide receptor. 4) Specific domains of the formyl peptide receptor were replaced by the same corresponding domain of the C5a receptor. Wild type and chimeric receptors were transfected into COS 7 cells and their ability to bind formyl peptide determined, taking into account efficiency of transfection and expression of chimeric protein. Based on these results, a ligand binding model is presented in which the second, third, and fourth extracellular (and/or their transmembrane) domains together with the first transmembrane domain form a ligand binding pocket for formyl peptides. It is proposed that the amino-terminal domain plays a role by presumably providing a "lid" to the pocket. The carboxyl-terminal cytoplasmic tail appears to modulate ligand binding by regulating receptor affinity.