N-acetylcysteine relieves oxidative stress and protects hippocampus of rat from radiation-induced apoptosis by inhibiting caspase-3

Abstract It has been recognized that radiation-induced effects remain a significant risk. An accumulation of reactive oxygen species (ROS) is considered to be one factor that contributes to neurodegenerative changes. The aim of our study was to investigate the potential radioprotective effects of NA...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2015-03, Vol.70, p.1-6
Main Authors: Li, Jianguo, Meng, Zhuangzhi, Zhang, Guowei, Xing, Yonghua, Feng, Li, Fan, Saijun, Fan, Feiyue, Buren, Batu, Liu, Qiang
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
Animals
Apoptosis - drug effects
Apoptosis - radiation effects
Caspase 3 - metabolism
Caspase Inhibitors - pharmacology
Caspase-3
Gamma Rays - adverse effects
Hippocampus
Hippocampus - cytology
Hippocampus - drug effects
Hippocampus - radiation effects
In Situ Nick-End Labeling
Internal Medicine
Male
Medical Education
N-acetylcysteine
Oxidative Stress - drug effects
Radiation
Random Allocation
Rats
Rats, Sprague-Dawley
Reactive oxygen species
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract It has been recognized that radiation-induced effects remain a significant risk. An accumulation of reactive oxygen species (ROS) is considered to be one factor that contributes to neurodegenerative changes. The aim of our study was to investigate the potential radioprotective effects of NAC. Male Sprague-Dawley rats underwent radiation. Irradiation was performed at room temperature with a 4-Gy dose of radiation. A dose of N-acetylcysteine (NAC) was performed 15 min prior to irradiation intraperitoneally. The methods of immunohistochemistry, TUNEL staining, Nissl staining, qRT-PCR, analysis of reactive oxygen species and Western blot were performed. In conclusion, our results demonstrate that NAC inhibits apoptosis induced by irradiation via the inhibition of caspase-3. We demonstrated a decrease in caspase-3 mRNA that was present at 24 h of NAC treatment. Such mRNA decrease was accompanied by a decrease of protein. In the present study, NAC effectively antagonized oxidation induced by irradiation. These results provide evidence that the neural protective effect and the antioxidant effect of NAC contribute to metabolic activity.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2014.12.029