Complete genome sequencing of Piper yellow mottle virus infecting black pepper, betelvine, and Indian long pepper

The complete genome of the Piper yellow mottle virus (PYMoV), a Badnavirus belonging to the family Caulimoviridae , was sequenced from three naturally infected hosts namely, black pepper, betelvine, and Indian long pepper. The genome length of the three virus strains (one from each of the three host...

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Bibliographic Details
Published in:Virus genes 2015-02, Vol.50 (1), p.172-175
Main Authors: Deeshma, K. P., Bhat, A. I.
Format: Article
Language:English
Subjects:
Badnavirus
Badnavirus - classification
Badnavirus - genetics
Badnavirus - isolation & purification
Biomedical and Life Sciences
Biomedicine
Cluster Analysis
DNA, Viral - chemistry
DNA, Viral - genetics
Genome, Viral
Medical Microbiology
Molecular Sequence Data
Molecular Weight
Open Reading Frames
Phylogeny
Piper - virology
Piper nigrum
Piper yellow mottle virus
Plant Sciences
Polyproteins - genetics
Sequence Analysis, DNA
Sequence Homology
Viral Proteins - chemistry
Viral Proteins - genetics
Virology
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Summary:The complete genome of the Piper yellow mottle virus (PYMoV), a Badnavirus belonging to the family Caulimoviridae , was sequenced from three naturally infected hosts namely, black pepper, betelvine, and Indian long pepper. The genome length of the three virus strains (one from each of the three host species) varied from 7,559 to 7,584 nucleotides, and all the three strains possessed four open reading frames (ORFs) I to IV that potentially encode proteins of 15.67, 17.08, 218.6, and 17.22 kDa, respectively. ORF III encodes a polyprotein consisting of viral movement protein, trimeric dUTPase, zinc finger, aspartic protease, reverse transcriptase, and RNase H whereas ORF I, II, and IV encode proteins of unknown functions. The complete genome sequences at the nucleotide level were 89–99 % identical with one available sequence of PYMoV and 39–56 % identical with other badnaviruses, indicating that all three are strains of PYMoV. Nucleotide and amino acid sequences of ORF I–IV and of the intergenic region (IR) were 80–100 % identical among PYMoV strains. Phylogenetic analysis of ORF III amino acid sequences showed the PYMoV strains forming a distinct cluster well separated from other badnaviruses. Among other badnaviruses, Fig badnavirus 1 (FBV-1) was the one most closely related to PYMoV.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-014-1134-0