Short and long-term antinutritional effect of the trypsin inhibitor ApTI for biological control of sugarcane borer

•Trypsin and chymotrypsin activities were strongly inhibited by ApTI.•The oviposition rate reduced by 43% in ApTI-fed larvae.•ApTI is refractory to digestion by D. saccharalis midgut proteases.•The effects exerted by ApTI are observed for consecutive generations. Plant-derived trypsin inhibitors hav...

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Bibliographic Details
Published in:Journal of insect physiology 2014-02, Vol.61, p.1-7
Main Authors: da Silva, Desireé S., de Oliveira, Caio F.R., Parra, José R.P., Marangoni, Sergio, Macedo, Maria L.R.
Format: Article
Language:English
Subjects:
Adaptation, Biological
Adenanthera pavonina
Animal Nutritional Physiological Phenomena - drug effects
Animals
Biological control
Diatraea saccharalis
Fabaceae - chemistry
Larva - drug effects
Moths - drug effects
Pest Control, Biological
Plant Proteins - pharmacology
Plant–insect interaction
Pupa - drug effects
Time Factors
Trypsin Inhibitors - pharmacology
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Summary:•Trypsin and chymotrypsin activities were strongly inhibited by ApTI.•The oviposition rate reduced by 43% in ApTI-fed larvae.•ApTI is refractory to digestion by D. saccharalis midgut proteases.•The effects exerted by ApTI are observed for consecutive generations. Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. In this work, we tested the anti-insect activity of Adenanthera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugarcane. The addition of 0.1% ApTI in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. ApTI was not digested after 60h incubation with D. saccharalis midgut proteases. The chronic effects of ApTI on F0 and F1 generations of D. saccharalis were also analyzed. The larvae from the F0 generation showed 55% and 21% decreased larval and pupal viability, respectively. ApTI-fed larvae from the F1 generation showed a decrease of 33% in survival rate and 23% in the average larval weight. Moreover, ApTI treatment reduced trypsin and chymotrypsin activities in F1 larvae. Thus, the anti-insect effects of ApTI on consecutive generations (F0 and F1) of D. saccharalis larvae demonstrate its potential for long-term control of this pest.
ISSN:0022-1910
1879-1611
DOI:10.1016/j.jinsphys.2013.11.012