Enhanced neuronal cell differentiation combining biomimetic peptides and a carbon nanotube-polymer scaffold

Abstract Carbon nanotubes are attractive candidates for the development of scaffolds able to support neuronal growth and differentiation thanks to their ability to conduct electrical stimuli, to interface with cells and to mimic the neural environment. We developed a biocompatible composite scaffold...

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Bibliographic Details
Published in:Nanomedicine 2015-04, Vol.11 (3), p.621-632
Main Authors: Scapin, Giorgia, MD, Salice, Patrizio, PhD, Tescari, Simone, MD, Menna, Enzo, PhD, De Filippis, Vincenzo, PhD, Filippini, Francesco, PhD
Format: Article
Language:English
Subjects:
Biomimetic Materials - chemistry
Biomimetic Materials - pharmacology
Biomimetic peptides
Carbon nanotube scaffold
Cell Differentiation - drug effects
Cell Line
Humans
Internal Medicine
Lactic Acid - chemistry
Lactic Acid - pharmacology
LINGO1
Membrane Proteins - chemistry
Membrane Proteins - pharmacology
Nanotubes, Carbon - chemistry
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - pharmacology
Neuronal differentiation
Neurons - cytology
Neurons - metabolism
Peptides - chemistry
Peptides - pharmacology
Polyesters
Polymers - chemistry
Polymers - pharmacology
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Summary:Abstract Carbon nanotubes are attractive candidates for the development of scaffolds able to support neuronal growth and differentiation thanks to their ability to conduct electrical stimuli, to interface with cells and to mimic the neural environment. We developed a biocompatible composite scaffold, consisting of multi-walled carbon nanotubes dispersed in a poly- l -lactic acid matrix able to support growth and differentiation of human neuronal cells. Moreover, to mimic guidance cues from the neural environment, we also designed synthetic peptides, derived from L1 and LINGO1 proteins. Such peptides could positively modulate neuronal differentiation, which is synergistically improved by the combination of the nanocomposite scaffold and the peptides, thus suggesting a prototype for the development of implants for long-term neuronal growth and differentiation. From the Clinical Editor The study describes the design and preparation of nanocomposite scaffolds with multi-walled carbon nanotubes in a poly-L-lactic acid matrix. This compound used in combination with peptides leads to synergistic effects in supporting neuronal cell growth and differentiation.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2014.11.001