Improving bone repair of femoral and radial defects in rabbit by incorporating PRP into PLGA/CPC composite scaffold with unidirectional pore structure

• Published in: Journal of biomedical materials research. Part A 2015-04, Vol.103 (4), p.1312-1324
• Main Authors: He, Fupo, Chen, Yan, Li, Jiyan, Lin, Bomiao, Ouyang, Yi, Yu, Bo, Xia, Yuanyou, Ye, Jiandong
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - metabolism; Animals; Biocompatibility; Biocompatible Materials - pharmacology; Biomedical materials; Bones; calcium phosphate cement; Calcium Phosphates - pharmacology; Cell Adhesion - drug effects; Cell Proliferation - drug effects; Cell Shape - drug effects; Defects; Femur - diagnostic imaging; Femur - drug effects; Femur - pathology; In vitro testing; Mesenchymal Stromal Cells - cytology; osteogenesis; platelet-rich plasma; Platelet-Rich Plasma - metabolism; Polyglycolic Acid - analogs & derivatives; Polyglycolic Acid - pharmacology; Porosity; Rabbits; Radius - diagnostic imaging; Radius - drug effects; Radius - pathology; Repair; Scaffolds; segmental bone defect; Surgical implants; Tissue Scaffolds - chemistry; unidirectional pore; Wound Healing - drug effects; X-Ray Microtomography
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35248

In this study, a platelet‐rich plasma poly(lactic‐co‐glycolic acid) (PRP‐PLGA)/calcium phosphate cement (CPC) composite scaffold was prepared by incorporating PRP into PLGA/CPC scaffold with unidirectional pore structure, which was fabricated by the unidirectional freeze casting of CPC slurry and the following infiltration of PLGA. The results from in vitro cell experiments and in vivo implantation in femoral defects manifested that incorporation of PRP into PLGA/CPC scaffold improved in vitro cell response (cell attachment, proliferation, and differentiation), and markedly boosted bone formation, angiogenesis and material degradation. The incorporation of PRP into scaffold showed more outstanding improvement in osteogenesis as the scaffolds were used to repair the segmental radial defects, especially at the early stage. The new bone tissues grew along the unidirectional lamellar pores of scaffold. At 12 weeks postimplantation, the segmental radial defects treated with PRP‐PLGA/CPC scaffold had almost recuperated, whereas treated with the scaffold without PRP was far from healed. Taken together, the PRP‐PLGA/CPC scaffold with unidirectional pore structure is a promising candidate to repair bone defects at various sites. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1312–1324, 2015.