Determination of polyphenolic constituents and biological activities of bark extracts from different Pinus species

BACKGROUND: The most common commercially available pine bark extract is Pycnogenol®, a standardised extract of Pinus maritima, which has been reported to have cardiovascular benefits and enhance microcirculation. The present study was conducted to determine the chemical composition of four pine bark...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2009-06, Vol.89 (8), p.1339-1345
Main Authors: Yesil-Celiktas, Ozlem, Ganzera, Markus, Akgun, Ismail, Sevimli, Canan, Korkmaz, Kemal S, Bedir, Erdal
Format: Article
Language:English
Subjects:
anticarcinogenic activity
antioxidant activity
Bark
Biological
Biological and medical sciences
breast neoplasms
catechin
cell lines
Cells
chemical composition
Comparative analysis
Constituents
cytotoxicity
Effects
Food industries
Foods
free radical scavengers
Fundamental and applied biological sciences. Psychology
Human
neoplasms
Pine
Pinus
Pinus brutia
Pinus nigra
Pinus pinea
Pinus sylvestris
plant extracts
polyphenols
prostatic neoplasms
Pycnogenol
Radicals
Scavenging
Studies
taxifolin
Trees
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Summary:BACKGROUND: The most common commercially available pine bark extract is Pycnogenol®, a standardised extract of Pinus maritima, which has been reported to have cardiovascular benefits and enhance microcirculation. The present study was conducted to determine the chemical composition of four pine bark extracts, assess their biological activities and to compare the results with Pycnogenol®. RESULTS: The Pinus species were analysed by LC and LC-MS; extracts of P. brutia and P. nigra showed higher levels of phenolic constituents compared to P. sylvestris and P. pinea. In particular, P. brutia contained extremely high concentrations of taxifolin (18.5%). The highest radical scavenging activities were attained with P. pinea (88.6%), P. nigra (87.2%) and P. brutia (86.4%) bark extracts. Additionally, anticarcinogenic effects of the extracts and their kinetics were determined in four cell lines including human prostate (PC-3, DU 145, LNCaP) and breast adenocarcinoma (MCF7) by the MTT assay. Cell viability was reduced to 40% by extracts of P. pinea, and P. sylvestris in PC-3 cells showing a similar effect like the positive control, CPT-11. CONCLUSION: Pinus species other than P. maritima definitively possess high biological activities, and therefore present a huge potential to be utilised in the food and the pharmaceutical industries.
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.3591