Benzylaminated dextran-modified hydrogels: A long-term bioactive TGF-[beta]1 carrier

Highly porous dextran-based hydrogels [in which various amounts (up to 16.6%, w/w) of a benzylaminated dextran (DMCB) exhibiting high affinity for TGF[beta]1 was immobilized] were developed to achieve long-term retention of bioactive TGF[beta]1 in situ. Unmodified hydrogels rapidly desorbed 80-90% c...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2009-12, Vol.91A (4), p.1178-1188
Main Authors: Degat, Marie-Christelle, Dahri-Correia, Latifa, Lavigne, Ferdinand, Meunier, Alain, Sedel, Laurent, Correia, Jose, Petite, Herve, Logeart-Avramoglou, Delphine
Format: Article
Language:English
Subjects:
Biomedical materials
Conjugation
Hydrogels
In vitro testing
Mathematical analysis
Matrices
Matrix methods
Surgical implants
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Summary:Highly porous dextran-based hydrogels [in which various amounts (up to 16.6%, w/w) of a benzylaminated dextran (DMCB) exhibiting high affinity for TGF[beta]1 was immobilized] were developed to achieve long-term retention of bioactive TGF[beta]1 in situ. Unmodified hydrogels rapidly desorbed 80-90% compared with only 40-60% of the preloaded TGF[beta]1 from the DMCB-modified hydrogels during a period of 21 days in PBS in vitro. TGF[beta]1 release experiments (performed with high ionic strength solutions) indicated that formation of the complex between TGF[beta]1 and functionalized hydrogels was governed by different interactions, depending on the degree of conjugation with DMCB: ionic interactions in the case of weakly conjugated matrices and nonionic interactions in highly conjugated matrices. Using cells containing a TGF[beta]-sensitive luciferase reporter gene, weakly DMCB-modified hydrogels sequestered bioactive TGF[beta]1 in situ, giving much higher, long-term signaling performance than highly functionalized hydrogels. Because these biocompatible functionalized hydrogels can provide long-term bioactive TGF[beta]1, they could be used as scaffolds for cells to stimulate and regulate human tissue repair processes.
ISSN:1552-4965
DOI:10.1002/jbm.a.32278