Extracellular fibril production by freshwater algae and cyanobacteria

• Published in: Microbial ecology 1992, Vol.23 (1), p.53-74
• Main Authors: Strycek, T. (University of Toronto, Toronto, Ontario, Canada), Acreman, J, Kerry, A, Leppard, G.G, Nermut, M.V, Kushner, D.J
• Format: Article
• Language: English
• Subjects: Acetates; ALGAE; ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; AZUCARES ACIDOS; Bacteriology; Biological and medical sciences; Cell walls; CRECIMIENTO; CROISSANCE; Cyanobacteria; CYANOPHYTA; Electron microscopy; FOSFATOS; Freshwater; Fundamental and applied biological sciences. Psychology; Materials; MEDIO DE CULTIVO; METHODE; METODOS; Micrasterias radiata; Microbiology; Microcystis aeruginosa; MILIEU DE CULTURE; Morphology, structure, chemical composition; MUCILAGE; MUCILAGOS; ONTARIO; PHOSPHATE; Phosphates; Ruthenium; Scenedesmus quadricauda; Solar fibrils; SUCRE ACIDE; Uronic acids; Xanthidium
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/BF00165907

In order to study the ability of freshwater algae and cyanobacteria to form extracellular fibrils, a screening test using ruthenium red (RR) staining was carried out on 28 species. Five of these were examined for growth and production of fibrillar material in culture media of different phosphate (P) contents. RR-staining and uronic acid determinations at various stages of algal growth were complemented by electron microscopy of the cells and of fibrillar material released into the medium. The lower P, concentrations enhanced growth of Micrasterias radiata, Eremosphaera sp., and Microcystis aeruginosa, and had little or no effect on growth of a Xanthidium sp. and Scenedesmus quadricauda. Extracellular uronic acid production, which was higher in low Pi medium in M. radiata, M. aeruginosa, and Xanthidium sp., could reach levels of 50 mg/liter or more. Algae with high proportions of RR-positive cells (M. radiata, Eremosphaera sp., Xanthidium sp., and M. aeruginosa) produced high levels of slime-like material and distinct fibrils that were often seen attached to the cell surface and only slowly released into the medium. No such material was found in cultures (or supernatants) of Sc. quadricauda, which also produced relatively low amounts of polyuronic acids. Specific types of filaments, often forming "fascicles" with rectangular arrays of globular particles were observed by negative staining electron microscopy of some algal cultures. RR-positive material was also observed in the cytoplasm and on the cell walls and surfaces of M. radiata and M. aeruginosa