Artificial proteins from combinatorial approaches

How do we create new artificial proteins? In this review, we present a range of experimental approaches based on combinatorial and directed evolution methods used to explore sequence space and recreate structured or active proteins. These approaches can help to understand constraints of natural evol...

Full description

Saved in:
Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2012-10, Vol.30 (10), p.512-520
Main Authors: Urvoas, Agathe, Valerio-Lepiniec, Marie, Minard, Philippe
Format: Article
Language:English
Subjects:
Amino acids
Assembly
Biological and medical sciences
Biotechnology
Biotechnology - methods
chemical reactions
Coalescence
Coalescing
Combinatorial analysis
Combinatorial Chemistry Techniques - methods
Deoxyribonucleic acid
directed evolution
Directed Molecular Evolution - methods
DNA
Enzymes
Evolution
Fragments
Fundamental and applied biological sciences. Psychology
Internal Medicine
Library collections
Methods. Procedures. Technologies
Polymerization
Polypeptides
Protein engineering
Protein folding
Proteins
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Strategy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:How do we create new artificial proteins? In this review, we present a range of experimental approaches based on combinatorial and directed evolution methods used to explore sequence space and recreate structured or active proteins. These approaches can help to understand constraints of natural evolution and can lead to new useful proteins. Strategies such as binary patterning or modular assembly can efficiently speed structural and functional innovation. Many natural protein architectures are symmetric or repeated and presumably have emerged by coalescence of simpler fragments. This process can be experimentally reproduced; a range of artificial proteins obtained from idealized fragments has recently been described and some of these have already found direct applications.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2012.06.001