Using surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture media

Yeastolate or yeast extract, which are hydrolysates produced by autolysis of yeast, are often employed as a raw material in the media used for industrial mammalian cell culture. The source and quality of yeastolate can significantly affect cell growth and production; however, analysis of these compl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Raman spectroscopy 2012-08, Vol.43 (8), p.1074-1082
Main Authors: Li, Boyan, Sirimuthu, Narayana M. S., Ray, Bryan H., Ryder, Alan G.
Format: Article
Language:English
Subjects:
Biotechnology
Discrimination
Fluorescence
fluorescence excitation-emission matrix spectroscopy
Hydrolysates
Media
Raman scattering
robust principal component analysis
Spectroscopy
surface-enhanced Raman scattering spectroscopy
Yeast
yeastolate/yeast extract
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Yeastolate or yeast extract, which are hydrolysates produced by autolysis of yeast, are often employed as a raw material in the media used for industrial mammalian cell culture. The source and quality of yeastolate can significantly affect cell growth and production; however, analysis of these complex biologically derived materials is not straightforward. The best current method, liquid chromatography–mass spectrometry (LC‐MS), is time‐consuming and requires extensive expertise. This study describes the use of surface‐enhanced Raman scattering (SERS) and fluorescence excitation–emission matrix (EEM) spectroscopy coupled with robust principal component analysis (ROBPCA) for the rapid and facile characterization and discrimination of yeast extracts in aqueous solution. SERS using silver colloids generates time‐dependent signals, where adenine is the strongest contributor, and the spectra are stable and reproducible (
ISSN:0377-0486
1097-4555
DOI:10.1002/jrs.3141