Aphanomyces species associated with epizootic ulcerative syndrome (EUS) in the Philippines and red spot disease (RSD) in Australia: preliminary comparative studies

Fungi morphologically consistent with class Oomycetes were recovered on primary culture from 20 of 22 ulcers on 21 fish with epizootic ulcerative syndrome (EUS) collected from 5 sites in the Philippines. Eleven primary isolates, and the unifungal cultures derived from them, were identified as Aphano...

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Bibliographic Details
Published in:Diseases of aquatic organisms 1995, Vol.21 (3), p.233-238
Main Authors: CALLINAN, R. B, PACLIBARE, J. O, BONDAD-REANTASO, M. G, CHIN, J. C, GOGOLEWSKI, R. P
Format: Article
Language:English
Subjects:
Animal mycoses
Biological and medical sciences
Freshwater
Infectious diseases
Medical sciences
Mycoses
Oomycetes
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Summary:Fungi morphologically consistent with class Oomycetes were recovered on primary culture from 20 of 22 ulcers on 21 fish with epizootic ulcerative syndrome (EUS) collected from 5 sites in the Philippines. Eleven primary isolates, and the unifungal cultures derived from them, were identified as Aphanomyces spp.; the remaining 9 primary isolates were lost through contaminant overgrowth. The Aphanomyces isolates were morphologically and culturally indistinguishable from those reported from red spot disease (RSD) in Australia. Comparison of 4 representative Aphanomyces isolates from Australian fish with RSD and 3 representative Aphanomyces isolates from Philippine fish with EUS, using SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis), revealed similar peptide banding profiles, indicative of a single Aphanomyces species. These findings, combined with epizootiological and pathological similarities between EUS and RSD, suggest the 2 syndromes are identical, and that a single Aphanomyces sp. may be the primary infectious cause.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao021233