Narirutin fraction from citrus peels attenuates LPS-stimulated inflammatory response through inhibition of NF-κB and MAPKs activation

• Published in: Food and chemical toxicology 2012-10, Vol.50 (10), p.3498-3504
• Main Authors: Ha, Sang Keun, Park, Ho-Young, Eom, Hyojin, Kim, Yoonsook, Choi, Inwook
• Format: Article
• Language: English
• Subjects: Animals; anti-inflammatory agents; Biological and medical sciences; Cell Line; Cell Survival - drug effects; Citrus; Citrus - chemistry; Citrus peel; citrus peels; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Cytokines - genetics; Cytokines - metabolism; dietary supplements; Dinoprostone - genetics; Dinoprostone - metabolism; Disaccharides - chemistry; Disaccharides - pharmacology; dose response; Flavanones - chemistry; Flavanones - pharmacology; Fruit - chemistry; Gene Expression Regulation, Enzymologic - drug effects; inducible nitric oxide synthase; Inflammation; interleukin-1beta; lipopolysaccharides; Lipopolysaccharides - toxicity; Macrophage; macrophages; Macrophages - drug effects; Macrophages - metabolism; Medical sciences; Mice; mitogen-activated protein kinase; Mitogen-Activated Protein Kinase Kinases - genetics; Mitogen-Activated Protein Kinase Kinases - metabolism; Molecular Structure; Narirutin; NF-kappa B - antagonists & inhibitors; NF-kappa B - genetics; NF-kappa B - metabolism; nitric oxide; Nitric Oxide - metabolism; Plant Extracts - chemistry; prostaglandin synthase; prostaglandins; Toxicology; transcription factor NF-kappa B; tumor necrosis factor-alpha
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2012.07.007

► Narirutin fraction from citrus peels inhibited LPS-induced inflammatory mediators. ► Narirutin fraction exerted anti-inflammatory effects by downregulating activity of MAPKs and NF-κB. ► Narirutin fraction may provide a potential therapeutic approach for inflammatory diseases. In this study, we examined the regulatory activity of narirutin fraction from citrus peels on the production of inflammatory mediators managing acute or chronic inflammatory diseases in macrophages. Narirutin fraction inhibited the release, by lipopolysaccharide (LPS)-stimulated macrophages, of nitric oxide (NO) and prostaglandin E2 (PGE2) through suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. The release, by LPS stimulated macrophages, of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was also reduced by narirutin fraction in a dose-dependent manner. Furthermore, narirutin fraction inhibited the LPS-mediated activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs), which are signaling molecules involved in production of pro-inflammatory factors. As a result of these properties, narirutin fraction has the potential to be used as a functional dietary supplement and effective anti-inflammatory agent.