A low-copy-number Sorghum DNA sequence that detects hypervariable EcoRV fragments

A sorghum genomic DNA clone that hybridized on Southern blots in simple but different patterns to fragments produced by digestion of DNA from the parents of an F2 mapping population was hybridized to EcoR5 digested DNA from 53 accessions. 46 different fragment patterns were observed, each comprised...

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Bibliographic Details
Published in:Theoretical and applied genetics 1994-09, Vol.89 (1), p.64-69
Main Authors: Cui, Y.X. (Texas A and M Univ., College Station (USA). Dept. of Plant Pathology and Microbiology), Xu, G.W, Magill, C.W, Schertz, K.F, Hart, G.E
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Fingerprinting
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Hybridization probe
Pteridophyta, spermatophyta
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
SORGHUM BICOLOR
VARIACION GENETICA
VARIATION GENETIQUE
Vegetals
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Summary:A sorghum genomic DNA clone that hybridized on Southern blots in simple but different patterns to fragments produced by digestion of DNA from the parents of an F2 mapping population was hybridized to EcoR5 digested DNA from 53 accessions. 46 different fragment patterns were observed, each comprised of from one to ten bands. Much less variability was detected in EcoR1 than EcoR5 digests of a selected subset of the accessions. Base-sequence analysis of the clone did not reveal a functional identity for the sequence and the clone does not contain repeated sequences often associated with hypervariable loci. Clones such as this will be especially useful in evaluating germplasm diversity and in identifying the potential parentage of hybrids.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226984