Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

Tracking stem cells in vivo using non-invasive techniques is critical to evaluate the efficacy and safety of stem cell therapies. Superparamagnetic iron oxide nanoparticles (SPIONs) enable cells to be tracked using magnetic resonance imaging (MRI), but to obtain detectable signal cells need to be la...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials science 2015-04, Vol.3 (4), p.608-616
Main Authors: Barrow, Michael, Taylor, Arthur, Nieves, Daniel J, Bogart, Lara K, Mandal, Pranab, Collins, Christopher M, Moore, Lee R, Chalmers, Jeffrey J, Lévy, Raphaël, Williams, Steve R, Murray, Patricia, Rosseinsky, Matthew J, Adams, Dave J
Format: Article
Language:English
Subjects:
Cell Tracking - methods
Coating
Contrast Media - chemistry
Dextrans - chemistry
Ferric Compounds - chemistry
Iron - chemistry
Iron oxides
Magnetic properties
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Magnetite Nanoparticles - chemistry
Metal Nanoparticles - chemistry
Stem Cells
Surface charge
Tracking
Uptakes
X-Ray Diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tracking stem cells in vivo using non-invasive techniques is critical to evaluate the efficacy and safety of stem cell therapies. Superparamagnetic iron oxide nanoparticles (SPIONs) enable cells to be tracked using magnetic resonance imaging (MRI), but to obtain detectable signal cells need to be labelled with a sufficient amount of iron oxide. For the majority of SPIONs, this can only be obtained with the use of transfection agents, which can adversely affect cell health. Here, we have synthesised a library of dextran-based polymer coated SPIONs with varying surface charge from -1.5 mV to +18.2 mV via a co-precipitation approach and investigated their ability to be directly internalised by stem cells without the need for transfection agents. The SPIONs were colloidally stable in physiological solutions. The crystalline phase of the particles was confirmed with powder X-ray diffraction and their magnetic properties were characterised using SQUID magnetometry and magnetic resonance. Increased surface charge led to six-fold increase in uptake of particles into stem cells and higher MRI contrast, with negligible change in cell viability. Cell tracking velocimetry was shown to be a more accurate method for predicting MRI contrast of stem cells compared to measuring iron oxide uptake through conventional bulk iron quantification.
ISSN:2047-4830
2047-4849
DOI:10.1039/c5bm00011d