Albusin B, mass-produced by the Saccharomyces cerevisiae suppression system, enhances lipid utilisation and antioxidant capacity in mice

• Published in: Journal of the science of food and agriculture 2013-08, Vol.93 (11), p.2758-2764
• Main Authors: Hsieh, Ya-Hui, Wang, Han-Tsung, Hsu, Jih-Tay, Chen, Ching-Yi
• Format: Article
• Language: English
• Subjects: Administration, Oral; albusin B; Animals; anti-atherosclerosis; antioxidant capacity; Antioxidants; Antioxidants - metabolism; Bacteriocins - chemistry; Bacteriocins - metabolism; Bacteriocins - pharmacology; Basal Metabolism; Fatty acids; Food science; Foods; Heart; Lipid Metabolism - drug effects; lipid utilisation; Lipids; Liver; Male; Metabolism; Mice; Mice, Inbred BALB C; Poultry; Random Allocation; Rodents; Ruminococcus albus; Saccharomyces cerevisiae; Yeast
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.6095

Background Albusin B (bacteriocin), isolated from Ruminococcus albus 7 and mass‐produced by the Saccharomyces cerevisiae expression system, has previously been shown to have a beneficial effect on lipid metabolism in broiler chickens. The present study was focused on the effect of albusin B on lipid metabolism in mice and the potential of albusin B‐expressing yeast product (albusin B) as a food supplement. Forty‐five BALB/c male mice at 6 weeks of age were each orally administered normal saline (control), yeast (0.125 mg kg−1) or albusin B (0.125 mg kg−1) for 14 days and then euthanised. Results Compared with the control group, albusin B‐fed mice exhibited decreased body weight and plasma levels of triglycerides and free fatty acids but increased plasma high‐density lipoprotein. Albusin B‐fed mice showed higher mRNA expression of fatty acid oxidation in the ileum, heart and liver than control mice. Compared with the control treatment, both yeast and albusin B treatments caused a decrease in mRNA expression of fatty acid synthesis in the heart and liver. Moreover, albusin B suppressed mRNA levels of lipogenesis in the ileum and liver. Albusin B‐fed mice exhibited more favourable adenosine triphosphate production and antioxidant capacity in the heart and liver. Albusin B treatment led to a significantly lower respiratory quotient than that of the control, whereas yeast treatment did not. Conclusion This study demonstrated a beneficial effect of albusin B on lipid utilisation and anti‐atherosclerotic and antioxidant capacities in mice. However, more comprehensive studies are required to elucidate the exact mechanism behind the effect of albusin B. © 2013 Society of Chemical Industry