An artificial fusion protein between bone morphogenetic protein 2 and titanium-binding peptide is functional in vivo

• Published in: Journal of biomedical materials research. Part A 2014-04, Vol.102 (4), p.1180-1186
• Main Authors: Yuasa, Kazuaki, Kokubu, Eitoyo, Kokubun, Katsutoshi, Matsuzaka, Kenichi, Shiba, Kiyotaka, Kashiwagi, Kenji, Inoue, Takashi
• Format: Article
• Language: English
• Subjects: Amino Acid Motifs; Animals; artificial protein; Biocompatibility; Biological and medical sciences; Biomedical materials; bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - pharmacology; Bones; Cartilage - drug effects; Cartilage - growth & development; Cartilage - metabolism; Chondrogenesis - drug effects; Chondrogenesis - genetics; Collagens; Core Binding Factor Alpha 1 Subunit - genetics; Core Binding Factor Alpha 1 Subunit - metabolism; Gene Expression Regulation - drug effects; in vivo; In vivo tests; Integrin-Binding Sialoprotein - genetics; Integrin-Binding Sialoprotein - metabolism; Medical sciences; Peptides - pharmacology; porous carbon; Proteins; Rats; Rats, Sprague-Dawley; Recombinant Fusion Proteins - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Scaffolds; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Tissue Scaffolds; Titanium; Titanium - pharmacology; titanium-binding peptide
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.34765

The purpose of this study was to investigate osteogenesis using an artificial fusion protein (AFP) composed of modified bone morphogenetic protein 2 (BMP‐2) with a titanium (Ti)‐binding peptide (TBP) motif on a Ti surface in vivo. In the in vivostudy, 5‐μm thick Ti was coated with electron cyclotron resonance sputtering on a porous carbon scaffold which was then dipped in one of three different mixtures of collagen gel: (1) collagen gel only, (2) collagen gel with TBP, and (3) collagen gel with the AFP between BMP‐2 and the TBP motif (AFP‐TBP–BMP‐2). These scaffolds were then implanted into rat abdominal muscles and were studied histologically at various times and the expression of several bone‐related protein messenger RNAs (mRNAs) was also analyzed. The Ti‐coated scaffold of the collagen gel with AFP‐TBP–BMP‐2 produced cartilage in the muscle and the expression of alkaline phosphatase, bone sialoprotein, and runt‐related gene 2 mRNAs was significantly increased. These results suggest that the scaffold of the collagen gel with AFP‐TBP–BMP‐2 accelerates osteogenesis in vivo. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1180–1186, 2014.