Histopathological, cytotoxicity and genotoxicity evaluation of Biosilicate registered glass-ceramic scaffolds

This study evaluated the biocompatibility of Biosilicate registered scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. The histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate registered , ev...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2013-03, Vol.101A (3), p.667-673
Main Authors: Kido, Hueliton W, Oliveira, Poliani, Parizotto, Nivaldo A, Crovace, Murilo C, Zanotto, Edgar D, Peitl-Filho, Oscar, Fernandes, Kristianne PS, Mesquita-Ferrari, Raquel A, Ribeiro, Daniel A, M Renno, Ana Claudia
Format: Article
Language:English
Subjects:
Biocompatibility
Biomaterials
Biomedical materials
Fibroblasts
Genotoxicity
Implantation
Osteoblasts
Scaffolds
Surgical implants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study evaluated the biocompatibility of Biosilicate registered scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. The histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate registered , evaluated at 7, 15, 30, 45, and 60 days after implantation. The cytotoxicity analysis was performed by the methyl thiazolyl tetrazolium (MTT) assay, with various concentrations of extracts from the biomaterial in culture of osteoblasts and fibroblasts after 24, 72, and 120 h. The genotoxicity analysis (comet assay) was performed in osteoblasts and fibroblasts after contact with the biomaterial during 24, 72, and 96 h. In the histopathology analysis, we observed a foreign body reaction, characterized by the presence of granulation tissue after 7 days of implantation of the biomaterial, and fibrosis connective tissue and multinucleated giant cells for longer periods. In the cytotoxicity analysis, extracts from the biomaterial did not inhibit the proliferation of osteoblasts and fibroblasts, and relatively low concentrations (12.5% and 25%) stimulated the proliferation of both cell types after 72 and 120 h. The analysis of genotoxicity showed that Biosilicate registered did not induce DNA damage in both lineages tested in all periods. The results showed that the Biosilicate registered scaffolds present in vivo and in vitro biocompatibility. copyright 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 667-673, 2013.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.34360