Protein-based nanobiosensor for direct detection of hydrogen sulfide

The chemically modified cytochrome c from equine heart, EC (232-700-9), was immobilized onto gold nanoparticles in order to develop a specific biosensing system for monitoring hydrogen sulfide down to the micromolar level, by means of a localized surface plasmon resonance spectroscopy. The sensing m...

Full description

Saved in:
Bibliographic Details
Published in:Europhysics letters 2015-01, Vol.109 (1), p.18005-p1-18005-p6
Main Authors: Omidi, Meisam, Amoabediny, Ghasem, Yazdian, Fatemeh, Habibi-Rezaei, M.
Format: Article
Language:English
Subjects:
78.67.Bf
87.14.E
87.85.fk
Biosensors
Cytochromes
Detection
Dielectric properties
Gold
Hydrogen sulfide
Monitoring
Nanoparticles
Nanostructure
Plasmons
Platforms
Red shift
Surface plasmon resonance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The chemically modified cytochrome c from equine heart, EC (232-700-9), was immobilized onto gold nanoparticles in order to develop a specific biosensing system for monitoring hydrogen sulfide down to the micromolar level, by means of a localized surface plasmon resonance spectroscopy. The sensing mechanism is based on the cytochrome-c conformational changes in the presence of H2S which alter the dielectric properties of the gold nanoparticles and the surface plasmon resonance peak undergoes a redshift. According to the experiments, it is revealed that H2S can be detected at a concentration of by the fabricated biosensor. This simple, quantitative and sensitive sensing platform provides a rapid and convenient detection for H2S at concentrations far below the hazardous limit.
ISSN:0295-5075
1286-4854
DOI:10.1209/0295-5075/109/18005