Caveolae-Mediated Endocytosis of Conjugated Polymer Nanoparticles

Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biologica...

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Bibliographic Details
Published in:Macromolecular bioscience 2013-07, Vol.13 (7), p.913-920
Main Authors: Lee, Junghan, Twomey, Megan, Machado, Christian, Gomez, Giselle, Doshi, Mona, Gesquiere, Andre J., Moon, Joong Ho
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Cancer
Carriers
Caveolae - chemistry
Caveolin 1 - chemistry
Caveolin 1 - metabolism
Cell Membrane Permeability - drug effects
Cells, Cultured
Cellular
conjugated polyelectrolytes
conjugated polymer nanoparticles
conjugated polymers
Degradation
Drug Delivery Systems
Endocytosis
endocytosis mechanism
Fluorescent Dyes - chemistry
Humans
Inhibitors
Nanoparticles
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Pathways
Polymers - administration & dosage
Polymers - chemistry
Proteins
small interfering RNA delivery
Uptakes
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Summary:Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae‐mediated endocytosis (CvME). CvME is further confirmed by high co‐localization with caveolin‐1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non‐toxicity, and non‐destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery. A detailed cellular entry mechanism of conjugated polymer nanoparticles (CPNs) is presented. Cancer cells pretreated with an inhibitor of caveolae‐mediated endocytosis (CvME) exhibit decreased CPN uptake. High co‐localization with caveolin‐1 proteins found in the caveolae and caveosomes further confirms CvME of CPNs. Non‐toxicity and non‐destructive delivery pathways support that CPNs are promising carriers, minimizing content degradation during delivery.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201300030