Sustained delivery of bioactive TGF- beta 1 from self-assembling peptide hydrogels induces chondrogenesis of encapsulated bone marrow stromal cells

Tissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti-inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor beta 1 (TGF- beta 1) from self-assembling peptide hy...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2014-05, Vol.102 (5), p.1275-1285
Main Authors: Kopesky, Paul W, Byun, Sangwon, Vanderploeg, Eric J, Kisiday, John D, Frisbie, David D, Grodzinsky, Alan J
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Bone marrow
Encapsulation
Hydrogels
Mathematical models
Peptides
Regenerative
Surgical implants
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Summary:Tissue engineering strategies for cartilage defect repair require technology for local targeted delivery of chondrogenic and anti-inflammatory factors. The objective of this study was to determine the release kinetics of transforming growth factor beta 1 (TGF- beta 1) from self-assembling peptide hydrogels, a candidate scaffold for cell transplant therapies, and stimulate chondrogenesis of encapsulated young equine bone marrow stromal cells (BMSCs). Although both peptide and agarose hydrogels retained TGF- beta 1, fivefold higher retention was found in peptide. Excess unlabeled TGF- beta 1 minimally displaced retained radiolabeled TGF- beta 1, demonstrating biologically relevant loading capacity for peptide hydrogels. The initial release from acellular peptide hydrogels was nearly threefold lower than agarose hydrogels, at 18% of loaded TGF- beta 1 through 3 days as compared to 48% for agarose. At day 21, cumulative release of TGF- beta 1 was 32-44% from acellular peptide hydrogels, but was 62% from peptide hydrogels with encapsulated BMSCs, likely due to cell-mediated TGF- beta 1 degradation and release of small labeled species. TGF- beta 1 loaded peptide hydrogels stimulated chondrogenesis of young equine BMSCs, a relevant preclinical model for treating injuries in young human cohorts. Self-assembling peptide hydrogels can be used to deliver chondrogenic factors to encapsulated cells making them a promising technology for in vivo, cell-based regenerative medicine. copyright 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1275-1285, 2014.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.34789