Reticulated bioactive scaffolds with improved textural properties for bone tissue engineering: Nanostructured surfaces and porosity

• Published in: Journal of biomedical materials research. Part A 2014-09, Vol.102 (9), p.2982-2992
• Main Authors: Ramiro-Gutiérrez, M. Lourdes, Will, Julia, Boccaccini, Aldo R., Díaz-Cuenca, Aránzazu
• Format: Article
• Language: English
• Subjects: bioactivity; Biocompatibility; Biological and medical sciences; Biotechnology; Bone Substitutes - chemistry; Bones; Ceramics - chemistry; Compressive strength; Delayed-Action Preparations - chemistry; dual macro nano-organised porous structure; enzyme carrier; Field emission; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Hydrogen-Ion Concentration; Industrial applications and implications. Economical aspects; Medical sciences; Miscellaneous; Muramidase - administration & dosage; Nanostructure; Nanostructures - chemistry; Nanostructures - ultrastructure; Porosity; reticulated scaffold; SBA-15 scaffolding processing; Scaffolds; Silicon dioxide; Silicon Dioxide - chemistry; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Three dimensional; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.34968

Organised nanoporous SBA‐15 type silica precursor (SP) particulate material has been processed into three‐dimensional macroporous, reticulated structures using a novel strategy consisting of blending increasing percentages of SP with a SiO2‐CaO‐P2O5 (80Si15Ca5P) mesoporous bioactive glass (MBG) sol. The procedure successfully produced consolidated and functionally competent open‐cell scaffolds while preserving the nanoporous order of the SP. Scaffolds were prepared using four different (MBG)/(SP) ratios. These structures were then characterized using field emission gun scanning electron microscopy, X‐ray diffraction (XRD), nitrogen adsorption‐desorption measurements, and compressive strength testing. Open‐cell interconnected structures with dual macro (150–500 μm) and nano (4–6 nm)‐organised porosity were produced. Both the textural and mechanical properties were found to improve with increasing SBA‐15 content. The in vitro bioactive response using simulated body fluid confirmed high reactivity for all prepared scaffolds. In addition, the SBA‐15 containing scaffolds exhibited a superior ability to delay the pH‐triggered lysozyme release with antibiotic activity. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2982–2992, 2014.