Hydroxyapatite nanocrystals functionalized with alendronate as bioactive components for bone implant coatings to decrease osteoclastic activity

•The bisphosphonate drug alendronate can be efficiently attached onto biomimetic hydroxyapatite nanocrystals.•Hydroxyapatite nanocrystals functionalized with alendronate significantly decrease osteoclastogenesis in RAW264.7 cells by inducing apoptosis.•Hydroxyapatite nanocrystals functionalized with...

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Bibliographic Details
Published in:Applied surface science 2015-02, Vol.328, p.516-524
Main Authors: Bosco, Ruggero, Iafisco, Michele, Tampieri, Anna, Jansen, John A., Leeuwenburgh, Sander C.G., van den Beucken, Jeroen J.J.P.
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Bisphosphonate
Bones
Coating
Coatings
Deposition
Electrospray
Hydroxyapatite
Nanocrystals
Osteoclast
Osteoporosis
Patients
Surgical implants
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Summary:•The bisphosphonate drug alendronate can be efficiently attached onto biomimetic hydroxyapatite nanocrystals.•Hydroxyapatite nanocrystals functionalized with alendronate significantly decrease osteoclastogenesis in RAW264.7 cells by inducing apoptosis.•Hydroxyapatite nanocrystals functionalized with alendronate can be simply and effectively deposited as a thin nanoscale coating on titanium using electrospray deposition. The integration of bone implants within native bone tissue depends on periprosthetic bone quality, which is severely decreased in osteoporotic patients. In this work, we have synthesized bone-like hydroxyapatite nanocrystals (nHA) using an acid–base neutralization reaction and analysed their physicochemical properties. Subsequently, we have functionalized the nHA with alendronate (nHAALE), a well-known bisphosphonate drug used for the treatment of osteoporosis. An in vitro osteoclastogenesis test was carried out to evaluate the effect of nHAALE on the formation of osteoclast-like cells from monocytic precursor cells (i.e. RAW264.7 cell line) showing that nHAALE significantly promoted apoptosis of osteoclast-like cells. Subsequently, nHA and nHAALE were deposited on titanium disks using electrospray deposition (ESD), for which characterisation of the deposited coatings confirmed the presence of alendronate in nHAALE coatings with nanoscale thickness of about 700nm. These results indicate that alendronate linked to hydroxyapatite nanocrystals has therapeutic potential and nHAALE can be considered as an appealing coating constituent material for orthopaedic and oral implants for application in osteoporotic patients.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2014.12.072