Clinical significance of HAX-1 expression in laryngeal carcinoma

• Published in: Auris, nasus, larynx nasus, larynx, 2015-08, Vol.42 (4), p.299-304
• Main Authors: You, Yiwen, Yao, Hao, You, Bo, Li, Xingyu, Ni, Haosheng, Shi, Si, Shan, Ying, Cao, Xiaolei
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Aged; Aged, 80 and over; Blotting, Western; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - surgery; Cohort Studies; Disease-Free Survival; Female; HAX-1; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - pathology; Head and Neck Neoplasms - surgery; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Laryngeal carcinoma; Laryngeal Neoplasms - genetics; Laryngeal Neoplasms - pathology; Laryngeal Neoplasms - surgery; Laryngectomy; Lymph Nodes - pathology; Male; Middle Aged; Neck Dissection; Neoplasm Staging; Otolaryngology; Prognosis; Proportional Hazards Models; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Squamous Cell Carcinoma of Head and Neck; Survival Rate; Two-step quantitative real-time PCR; Western blot
• ISSN: 0385-8146; 1879-1476
• DOI: 10.1016/j.anl.2014.12.003

Abstract Objective HS1-associated protein X-1 (HAX-1) is a multifunctional protein that has been highlighted as an important marker in many types of cancers. However, little is known about the role of HAX-1 in laryngeal carcinoma. The purpose of the present study is to explore HAX-1 expression status and its associations with clinicopathologic features and survival in a well-defined cohort of laryngeal carcinoma. Methods We examined the expression of HAX-1 at protein and mRNA levels in laryngeal carcinoma tissues and adjacent non-tumor tissues by immunohistochemistry, Western blotting and two-step quantitative real-time PCR analysis, respectively. Results We observed that HAX-1 was significantly elevated in laryngeal carcinoma. The relationship between the levels of HAX-1 expression and clinicopathologic characteristics was then analyzed. Overexpression of HAX-1 was significantly correlated with T classification, lymph node metastasis, clinical stage, and pathology. Survival curves were plotted using the Kaplan–Meier method and compared using the log-rank test. We find that patients with overexpression of HAX-1 had shorter overall survival rates. Finally, the significance of various survival variables was analyzed using multivariate Cox proportional hazards model. We found that overexpression of HAX-1 was an independent prognostic factor for patients with laryngeal carcinoma. Conclusion Our findings hinted that overexpression of HAX-1 was a potentially unfavorable factor in the progression and prognosis of laryngeal carcinoma.