High force development augments skeletal muscle signalling in resistance exercise modes equalized for time under tension

How force development and time under tension (TUT) during resistance exercise (RE) influence anabolic signalling of skeletal muscle is incompletely understood. We hypothesized that high force development during RE is more important for post-exercise-induced signalling than submaximal and fatiguing R...

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Bibliographic Details
Published in:Pflügers Archiv 2015-06, Vol.467 (6), p.1343-1356
Main Authors: Gehlert, Sebastian, Suhr, Frank, Gutsche, Katrin, Willkomm, Lena, Kern, Julia, Jacko, Daniel, Knicker, Axel, Schiffer, Thorsten, Wackerhage, Henning, Bloch, Wilhelm
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - metabolism
Adult
Biomedical and Life Sciences
Biomedicine
Cell Biology
Focal Adhesion Kinase 2 - metabolism
Human Physiology
Humans
Male
MAP Kinase Kinase 4 - metabolism
Molecular Medicine
Muscle Contraction
Muscle Fatigue
Muscle Physiology
Muscle Strength
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiology
Neurosciences
Phosphoproteins - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Receptors
Resistance Training
Ribosomal Protein S6 Kinases, 70-kDa - metabolism
Signal Transduction
TOR Serine-Threonine Kinases - metabolism
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Summary:How force development and time under tension (TUT) during resistance exercise (RE) influence anabolic signalling of skeletal muscle is incompletely understood. We hypothesized that high force development during RE is more important for post-exercise-induced signalling than submaximal and fatiguing RE with lower force development but similar TUT. Twenty-two male subjects (24 ± 6 years, 181 ± 9 cm, 79 ± 2 kg) performed three distinct RE modes in the fed state with equal TUT but distinct force output: (i) maximal eccentric RE (ECC, n  = 7) three sets, eight reps, 100 % eccentric dynamic force; (ii) standard RE (STD, n  = 7), three sets, 10 reps, 75 % dynamic force; and (iii) high fatiguing single-set RE (HIT, n  = 8), 20 reps, 100 % eccentric-concentric force; vastus lateralis biopsies were collected at baseline, 15, 30, 60, 240  min and 24  h after RE, and the signalling of mechanosensitive and mammalian target of rapamycin (mTOR)-related proteins was determined. The phosphorylation levels of pFAK Tyr397 , pJNK Thr183/Tyr185 , pAKT Thr308/Ser473 , pmTOR Ser2448 , p4E-BP1 Thr37/46 , p70s6k Thr389 / Ser421/Thr424 and pS6 Ser235/236 were significantly higher in ECC than those in STD and HIT at several time points ( P  
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-014-1579-y