Inhibition of a conjugation-like gene transfer process in Bacillus thuringiensis subsp. israelensis by the anti-S-layer protein antibody

Extraction of the S-layer protein by treatment with 6 M urea revealed a high-molecularweight protein in the extracts obtained from Bacillus thuringiensis subsp. israelensis (B.t.i) strain 4Q2. This protein band was found to be absent in partially cured (4Q2-72) and completely cured (c4Q2-72) strains...

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Bibliographic Details
Published in:Current microbiology 1995-02, Vol.30 (2), p.69-75
Main Authors: Wiwat, C. (Mahidol University, Bangkok, Thailand.), Panbangred, W, Mongkolsuk, S, Pantuwatana, S, Bhumiratana, A
Format: Article
Language:English
Subjects:
ANTICORPS
ANTICUERPOS
BACILLUS THURINGIENSIS
Bacillus thuringiensis israelensis
BACTERIA
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genetics
INHIBICION
INHIBITION
Microbiology
PLASMIDE
PLASMIDIOS
PROTEINAS
PROTEINE
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
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Summary:Extraction of the S-layer protein by treatment with 6 M urea revealed a high-molecularweight protein in the extracts obtained from Bacillus thuringiensis subsp. israelensis (B.t.i) strain 4Q2. This protein band was found to be absent in partially cured (4Q2-72) and completely cured (c4Q2-72) strains. The antibody toward this S-layer protein was prepared and used to locate its antigenic protein on B.t.i. cells by using indirect immunofluorescence. Immunodiffusion reactions and Western blot analysis confirmed the specificity of the anti-S-layer protein antibody. It was found that the antibody against 4Q2 S-layer protein inhibited plasmid transfer via a conjugation-like process between B.t.i. strains 4Q2-16 and c4Q2-72. That is, the frequency of transfer of plasmid pBC16 was reduced from 9.7 X 10(-6) in the absence of the antibody to less than 1.0 X 10(-8) in the presence of the antibody. The antibody was also found to reduce the frequency of pBC16 plasmid transfer via a conjugation-like process between B.t.i. strains A084-16-194 and c4Q2-72 from 2.2 X 10(-5) in the absence of the antibody to 1.2 X 10(-6) in the presence of the antibody
ISSN:0343-8651
1432-0991
DOI:10.1007/BF00294185