Reduction in the amount of 8-hydroxy-2'-deoxyguanosine in the DNA of SV40-transformed human fibroblasts as compared with normal cells in culture

DNA damage due to oxidative free radicals is considered to be a major cause of ageing and age-related diseases including cancer. Of more than 20 modifications formed in DNA by the action of hydroxyl radicals, 8-hydroxy-2'-deoxyguanosine (oh 8dG) is potentially highly mutagenic and is known to o...

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Bibliographic Details
Published in:FEBS letters 1993-03, Vol.318 (2), p.186-188
Main Authors: Barciszewski, Jan, Rattan, Suresh I.S., Siboska, Gunhild E., Otzen, Daniel E., Clark, Brian F.C.
Format: Article
Language:English
Subjects:
8-Hydroxydeoxyguanosine
Biological and medical sciences
Cell Transformation, Viral
Cells, Cultured
Chromatography, High Pressure Liquid
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - chemistry
DNA Damage
DNA Repair
Free Radicals
Fundamental and applied biological sciences. Psychology
HPLC/EC
Humans
In Vitro Techniques
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Oxidative DNA damage
Simian virus 40
Transformed cell
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Summary:DNA damage due to oxidative free radicals is considered to be a major cause of ageing and age-related diseases including cancer. Of more than 20 modifications formed in DNA by the action of hydroxyl radicals, 8-hydroxy-2'-deoxyguanosine (oh 8dG) is potentially highly mutagenic and is known to occur most frequently. Using HPLC combined with electrochemical (HPLC/EC) detection of oh 8dG, fivefold higher levels of oh 8dG are detected in the DNA of cultured normal human skin fibroblasts as compared with SV40-transformed human fibroblasts MRC-5V2. In comparison, the levels of oh 8dG were similar in the growth medium of both types of cells. Applications of this method range from studies on the genomic stability and instability of normal and cancerous cells to the clinical and laboratory testing of toxic substances and drugs.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80018-P