Acid environments affect biofilm formation and gene expression in isolates of Salmonella enterica Typhimurium DT104

• Published in: International journal of food microbiology 2015-08, Vol.206, p.7-16
• Main Authors: O'Leary, Denis, McCabe, Evonne M., McCusker, Matthew P., Martins, Marta, Fanning, Séamus, Duffy, Geraldine
• Format: Article
• Language: English
• Subjects: Acids - pharmacology; Antibiotic resistance; Biofilm; Biofilms; Food Microbiology; Gene expression; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - physiology; Hydrogen-Ion Concentration; Microbial Viability - drug effects; Salmonella enterica; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Salmonella typhimurium - physiology; Stainless Steel; Stress, Physiological - drug effects; Virulence - genetics
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2015.03.030

The aim of this study was to examine the survival and potential virulence of biofilm-forming Salmonella Typhimurium DT104 under mild acid conditions. Salmonella Typhimurium DT104 employs an acid tolerance response (ATR) allowing it to adapt to acidic environments. The threat that these acid adapted cells pose to food safety could be enhanced if they also produce biofilms in acidic conditions. The cells were acid-adapted by culturing them in 1% glucose and their ability to form biofilms on stainless steel and on the surface of Luria Bertani (LB) broth at pH7 and pH5 was examined. Plate counts were performed to examine cell survival. RNA was isolated from cells to examine changes in the expression of genes associated with virulence, invasion, biofilm formation and global gene regulation in response to acid stress. Of the 4 isolates that were examined only one (1481) that produced a rigid biofilm in LB broth at pH7 also formed this same structure at pH5. This indicated that the lactic acid severely impeded the biofilm producing capabilities of the other isolates examined under these conditions. Isolate 1481 also had higher expression of genes associated with virulence (hilA) and invasion (invA) with a 24.34-fold and 13.68-fold increase in relative gene expression respectively at pH5 compared to pH7. Although genes associated with biofilm formation had increased expression in response to acid stress for all the isolates this only resulted in the formation of a biofilm by isolate 1481. This suggests that in addition to the range of genes associated with biofilm production at neutral pH, there are genes whose protein products specifically aid in biofilm production in acidic environments. Furthermore, it highlights the potential for the use of lactic acid for the inhibition of Salmonella biofilms. •The response of Salmonella Typhimurium to acid stress was analysed.•Lactic acid impeded biofilm growth in Salmonella isolates.•Upregulation of virulence and invasion genes for biofilm formed at pH5 vs. pH7•Specific gene products responsible for biofilm formation in acidic conditions