Quantification of 2,4-D and related chlorophenoxy herbicides by a magnetic particle-based ELISA

• Published in: Bulletin of environmental contamination and toxicology 1994-04, Vol.52 (4), p.538-545
• Main Authors: Lawruk, T.S, Hottenstein, C.S, Fleeker, J.R, Hall, J.C, Herzog, D.P, Rubio, F.M
• Format: Article
• Language: English
• Subjects: 2,4-D; 2,4-Dichlorophenoxyacetic Acid - analysis; AGUA; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; Analytical chemistry; Chemistry; Contamination; Desorption; EAU; ELISA; Enzyme-Linked Immunosorbent Assay - methods; ESTER; ESTERES; ESTERS; Exact sciences and technology; HERBICIDAS; HERBICIDE; HERBICIDES; Herbicides - analysis; Miscellaneous; POLLUTION DE L'EAU; POLUCION DEL AGUA; Q1; QUANTITATIVE ANALYSIS; Runoff; Sensitivity and Specificity; TEST ELISA; WATER; Water Pollutants, Chemical - analysis; WATER POLLUTION; Water resources
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/BF00194141

The development of a magnetic particle based ELISA for 2,4-D is described. The assay sensitivity, based on the concentration producing 90 per cent of the light absorbance at 450 nm of a zero standard was 0.7 ppb. Recovery of a reference standard, was an average of 98 per cent across a concentration range, and the accuracy was linear. Groundwater samples assayed by this ELISA and an established gas chromatography/mass spectroscopy method gave a correlation of 0.970 and a slope of 0.938 between methods. Specificity data for a variety of chlorophenoxy analogues and unrelated chemicals showed that the ELISA was up to 20 times more sensitive to esters of 2,4-D. Other groundwater components did not interfere with the assay at concentrations higher than usually found. The assay was fast, sensitive and accurate and appeared suitable for monitoring 2,4-D in water.